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Naphthol AS-BI-Phosphate Sale

(Synonyms: 萘酚AS-BI磷酸盐) 目录号 : GC44314

A fluorogenic substrate for acid and alkaline phosphatases

Naphthol AS-BI-Phosphate Chemical Structure

Cas No.:1919-91-1

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Sample solution is provided at 25 µL, 10mM.

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产品描述

Naphthol AS-BI-phosphate is a fluorogenic substrate for acid and alkaline phosphatases. Naphthol AS-BI-phosphate is converted to naphthol AS-BI (N-ASBI) which displays excitation/emission spectra of 405/515 nm, respectively. N-ASBI fluorescence is a quantitative marker of acid and alkaline phosphatase activity. The concentration of human acid and alkaline phosphatases undergo pronounced changes in particular diseases, resulting in unusually high or low concentrations. Thus, acid and alkaline phosphatases are often used as clinical markers of disease.

Chemical Properties

Cas No. 1919-91-1 SDF
别名 萘酚AS-BI磷酸盐
Canonical SMILES BrC1=CC=C(C=C(OP(O)(O)=O)C(C(NC2=C(OC)C=CC=C2)=O)=C3)C3=C1
分子式 C18H15BrNO6P 分子量 452.2
溶解度 DMF: 20 mg/ml,DMSO: 20 mg/ml,DMSO:PBS(pH 7.2) (1:3): 0.25 mg/ml,Ethanol: 2 mg/ml 储存条件 Store at -20°C
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1 mg 5 mg 10 mg
1 mM 2.2114 mL 11.0571 mL 22.1141 mL
5 mM 0.4423 mL 2.2114 mL 4.4228 mL
10 mM 0.2211 mL 1.1057 mL 2.2114 mL
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Research Update

One-step double immunolabeling of mouse interdigitating reticular cells: simultaneous application of pre-formed complexes of monoclonal rat antibody M1-8 with horseradish peroxidase-linked anti-rat immunoglobulins and of monoclonal mouse anti-Ia antibody with alkaline phosphatase-coupled anti-mouse immunoglobulins

J Histochem Cytochem 1991 Dec;39(12):1719-23.PMID:1940324DOI:10.1177/39.12.1940324.

A novel one-step double immunolabeling method was elaborated on the basis of the simultaneous application of preformed molecular complexes of two primary antibodies with their specific secondary antibodies labeled with different enzymes. Treatment with a rat monoclonal antibody (MAb), M1-8, pre-coupled with horseradish peroxidase-linked sheep anti-rat immunoglobulins, and enzyme reaction revealed by the 3-amino-9-ethylcarbazole/hydrogen peroxide reaction, resulted in red-brown intracytoplasmic staining of interdigitating reticular cells in the lymph nodes of Balb/c mice. Another molecular complex, made of mouse anti-Ia MAb with alkaline phosphatase-linked rabbit anti-mouse immunoglobulins, applied at the same time and then developed with Naphthol AS-BI-Phosphate/fast blue BB as substrate, yielded blue surface staining of this cell type in addition to labeling of B-lymphocytes. The method described provides the possibility of relatively rapid double antigen detection where the binding sites of the secondary antibodies are saturated by the specific primary immunoglobulins. This approach seems to avoid nonspecific binding of primary antibodies to Fc receptors, and the unwanted binding of secondary antibodies with cell surface immunoglobulins on B-lymphocytes or with crossreactive primary antibodies used in the other sequence, if the primary antibodies and the tissue are the same or crossreactive animal species.