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NAI-N3 Sale

目录号 : GC30021

NAI-N3是新型的RNA酰化试剂,用来进行RNA纯化。

NAI-N3 Chemical Structure

Cas No.:1612756-29-2

规格 价格 库存 购买数量
5mg
¥1,620.00
现货
10mg
¥2,610.00
现货

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Sample solution is provided at 25 µL, 10mM.

产品文档

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产品描述

NAI-N3 is a novel RNA acylation reagent that enables RNA purification.

The bifunctional NAI-N3 SHAPE reagent is used to label all RNAs inside living cells (or in vitro refolded RNA) to generate covalent modifications on targeted RNAs at single-nucleotide resolution. RNA is isolated, biotinylated using click chemistry and randomly fragmented to a size of 50-100 nt. To generate cDNA sequencing libraries, the RNA fragments are subjected to 3′-end repair, adapter ligation, size selection, biotin enrichment, reverse transcription (RT) and PCR. The end result is selective amplification of fragments corresponding to modified bases in the NAI-N3-treated samples or background expression values from mock-treated samples. Sequencing reads generated from the NAI-N3- and mock (DMSO)-treated samples are mapped to a transcriptome annotation, followed by computational identification of RT stops corresponding to SHAPE modifications—and hence reactive nucleotides—across the entire transcriptome[1].

[1]. Flynn RA, et al. Transcriptome-wide interrogation of RNA secondary structure in living cells with icSHAPE. Nat Protoc. 2016 Feb;11(2):273-90.

Chemical Properties

Cas No. 1612756-29-2 SDF
Canonical SMILES O=C(C1=CC=CN=C1CN=[N+]=[N-])N2C=CN=C2
分子式 C10H8N6O 分子量 228.21
溶解度 DMSO : ≥ 115 mg/mL (503.92 mM);Water : 1 mg/mL (4.38 mM) 储存条件 Store at -20°C(sealed storage, away from moisture)
General tips 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

溶解性数据

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1 mg 5 mg 10 mg
1 mM 4.3819 mL 21.9096 mL 43.8193 mL
5 mM 0.8764 mL 4.3819 mL 8.7639 mL
10 mM 0.4382 mL 2.191 mL 4.3819 mL
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