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Leukotriene B4-d4 Sale

(Synonyms: LTB4-d4; 5(S),12(R)-DiHETE-d4) 目录号 : GC47556

An internal standard for the quantification of leukotriene B4

Leukotriene B4-d4 Chemical Structure

Cas No.:124629-74-9

规格 价格 库存 购买数量
25 μg
¥4,300.00
现货
100 μg
¥10,758.00
现货

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Sample solution is provided at 25 µL, 10mM.

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产品描述

Leukotriene B4-d4 (LTB4-d4) contains four deuterium atoms at the 6, 7, 14, and 15 positions. It is intended for use as an internal standard for the quantification of LTB4 by GC- or LC-mass spectrometry. LTB4 is a dihydroxy fatty acid derived from arachidonic acid through the 5-lipoxygenase pathway.1,2,3 It promotes a number of leukocyte functions including aggregation, stimulation of ion fluxes, enhancement of lysosomal enzyme release, superoxide anion production, chemotaxis, and chemokinesis. In subnanomolar ranges (3.9 x 10-10 M), LTB4 causes chemotaxis and chemokinesis in human PMNL.4 At higher concentrations, (1.0 x 10-7 M), LTB4 leads to neutrophil aggregation and degranulation as well as superoxide anion production.4,5

1.RÅdmark, O., Malmsten, C., Samuelsson, B., et al.Leukotriene A: Stereochemistry and enzymatic conversion to leukotriene BBiochem. Biophys. Res. Commun.92(3)954-961(1980) 2.Ford-Hutchinson, A.W., Bray, M.A., Doig, M.V., et al.Leukotriene B, a potent chemokinetic and aggregating substance released from polymorphonuclear leukocytesNature286(5770)264-265(1980) 3.McGee, J., and Fitzpatrick, F.Enzymatic hydration of leukotriene A4. Purification and characterization of a novel epoxide hydrolase from human erythrocytesJ. Biol. Chem.260(23)12832-12837(1985) 4.Ford-Hutchinson, A.W.Leukotriene B4 in inflammationCrit. Rev. Immunol.10(1)1-12(1990) 5.McMillan, R.M., and Foster, S.J.Leukotriene B4 and inflammatory diseaseAgents Actions24(1-2)114-119(1988)

Chemical Properties

Cas No. 124629-74-9 SDF
别名 LTB4-d4; 5(S),12(R)-DiHETE-d4
Canonical SMILES CCCCC/C=C\C[C@@H](O)/C=C/C=C\C=C\[C@@H](O)CCCC(=O)O
分子式 C20H28D4O4 分子量 340.5
溶解度 DMF: >50 mg/ml (per Rao Maddipati),DMSO: >50 mg/ml (per Rao Maddipati),Ethanol: >50 mg/ml (per Rao Maddipati),PBS pH 7.2: >1 mg/ml (from 13(S)-HODE) 储存条件 Store at -20°C
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1 mM 2.9369 mL 14.6843 mL 29.3686 mL
5 mM 0.5874 mL 2.9369 mL 5.8737 mL
10 mM 0.2937 mL 1.4684 mL 2.9369 mL
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Research Update

A highly sensitive and selective method for the determination of leukotriene B4 (LTB4) in ex vivo stimulated human plasma by ultra fast liquid chromatography-tandem mass spectrometry

J Chromatogr B Analyt Technol Biomed Life Sci 2013 Apr 15;925:54-62.PMID:23523878DOI:10.1016/j.jchromb.2013.02.038

Leukotriene B4 (LTB4) is an important inflammatory component in a number of diseases and has been used as a pharmacodynamic (PD) biomarker. In this report, a highly sensitive and selective ultra fast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) method for the determination of LTB4 in plasma from ex vivo stimulated human blood, using Leukotriene B4-d4 (LTB4-d4, contains four deuterium atoms at the 6, 7, 14, and 15 positions) as the internal standard (IS), was developed and validated. The chromatographic separation of LTB4 from its three isomers and an unknown interference peak from human plasma was crucial to achieve accurate determination of 0.2 ng/mL (LLOQ) of LTB4. LTB4 and the IS were extracted with methyl tertiary butyl ether (MTBE) from 200 μL human plasma. Reversed-phase HPLC separation was carried out with a Phenomenex Synergi Hydro-RP column (100mm×3mm, 2.5 μm). MS/MS detection was set at mass transitions of 335.0→194.9 m/z for LTB4 and 339.0→196.9 m/z for LTB4-d4 in Turbo Ionization Spray (TIS) negative mode. The dynamic range of the method is 0.2-200 ng/mL. LTB4 was found to be stable in human plasma for at least three freeze (-20 °C)/thaw cycles, and on the benchtop (room temperature) for at least 6h. The stock solution storage stability study demonstrated that the LTB4 stock solution, in 50:50 acetonitrile:water, was stable at 4 °C for at least 198 days. The processed samples were found to be stable for at least 72 h at room temperature. The long-term sample storage stability test demonstrated that LTB4 human plasma samples were stable at a storage temperature of -20 °C for at least 198 days. In addition, intraday and interday accuracy and precision, sensitivity, linearity, and recovery were evaluated. An additional partial validation was conducted to decrease the plasma sample volume from 200 to 100 μL. All the data reported in this study fulfilled the requirements and recommendations in the FDA guidance for bioanalytical method validation. Comparison of the validated UFLC-MS/MS method with an ELISA method using ex vivo stimulated samples indicated that although results from the two assays correlated relatively well, the UFLC-MS/MS method has been shown to be superior in selectivity and dynamic range to an ELISA method in our study. The validated UFLC-MS/MS method was successfully used to analyze samples generated from two clinical studies. The excellent assay performance and incurred sample reproducibility (ISR) results obtained from the study sample analysis demonstrated the assay is robust and reliable.