Lecithin
(Synonyms: 卵磷脂) 目录号 : GC30103
A sphingolipid
Cas No.:8002-43-5
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Cell experiment: |
MRS broths are supplemented with soy Lecithin concentrations of 0, 0.2, 0.4, 0.6, 0.8 and 1.0%. Each broth is inoculated with a tested strain culture (2%, v/v) and anaerobically incubated at 37°C for 20 h. After incubation, the bacterium cells are harvested by centrifugation at 8000 g for 10 min at 4°C and washed twice in PBS (pH 6.5) plus ethanol (5%, v/v). Strain bile resistance is assessed. The numbers of viable cells are counted by the pouring plate method, and each batch is tested three times[1]. |
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References: [1]. Hu B, et al. Enhancement of bile resistance in Lactobacillus plantarum strains by soy lecithin. Lett Appl Microbiol. 2015 Jul;61(1):13-9. |
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Phosphatidylcholine is the most abundant phospholipid in mammalian, plant, and yeast cells.1 It is found mainly in the outer leaflet of cell membranes and can make up approximately half of the total phospholipids.2 In mammalian tissues, phosphatidylcholine commonly contains a saturated and unsaturated fatty acid at the C-1 and C-2 positions of glycerol, respectively. It is a substrate for various enzymes in cell signaling pathways that is cleaved by phospholipases into diacylglycerol and phosphocholine or phosphatidic acid and choline. This product contains glucosylceramide molecular species with primarily C16:0, C22:0, and C24:0 fatty acyl chain lengths. As this product is derived from a natural source, there may be variations in the sphingoid backbone. [Matreya, LLC. Catalog No. 1044]
1.Vance, J.E.Phosphatidylserine and phosphatidylethanolamine in mammalian cells: Two metabolically related aminophospholipidsJ. Lipid Res.49(7)1377-1387(2008) 2.Billah, M.M., and Anthes, J.C.The regulation and cellular functions of phosphatidylcholine hydrolysisBiochem J.269(2)281-291(1990)
| Cas No. | 8002-43-5 | SDF | |
| 别名 | 卵磷脂 | ||
| Canonical SMILES | O=P(OC[C@@H](COC(CCCCCCCCCCCCCCC)=O)OC(CCCCCCC/C=C\C/C=C\CCCCC)=O)(OCC[N+](C)(C)C)[O-] | ||
| 分子式 | C42H80NO8P | 分子量 | 758.06 |
| 溶解度 | DMSO : 5 mg/mL (6.60 mM);Water : 3.33 mg/mL (4.39 mM) | 储存条件 | Store at 2-8°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.3192 mL | 6.5958 mL | 13.1916 mL |
| 5 mM | 0.2638 mL | 1.3192 mL | 2.6383 mL |
| 10 mM | 0.1319 mL | 0.6596 mL | 1.3192 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Lecithin-based nanostructured gels for skin delivery: an update on state of art and recent applications
J Control Release 2014 Apr 28;180:10-24.24531009 10.1016/j.jconrel.2014.02.004
Conventional carriers for skin delivery encounter obstacles of drug leakage, scanty permeation and low entrapment efficiency. Phospholipid nanogels have recently been recognized as prominent delivery systems to circumvent such obstacles and impart easier application. The current review provides an overview on different types of Lecithin nanostructured gels, with particular emphasis on liposomal versus microemulsion gelled systems. Liposomal gels investigated encompassed classic liposomal hydrogel, modified liposomal gels (e.g. Transferosomal, Ethosomal, Pro-liposomal and Phytosomal gels), Microgel in liposomes (M-i-L) and Vesicular phospholipid gel (VPG). Microemulsion gelled systems encompassed Lecithin microemulsion-based organogels (LMBGs), Pluronic Lecithin organogels (PLOs) and Lecithin-stabilized microemulsion-based hydrogels. All systems were reviewed regarding matrix composition, state of art, characterization and updated applications. Different classes of Lecithin nanogels exhibited crucial impact on transdermal delivery regarding drug permeation, drug loading and stability aspects. Future perspectives of this theme issue are discussed based on current laboratory studies.
Lecithin as a therapeutic agent in ulcerative colitis
Dig Dis 2013;31(3-4):388-90.24246994 10.1159/000354707
Lecithin [phosphatidylcholine (PC)] was shown to account for more than 70% of total phospholipids within the intestinal mucus layer. It is arranged in lamellar membranes (surfactant-like particles) and establishes a hydrophobic barrier preventing invasion of the colonic commensal microbiota. In ulcerative colitis (UC), the mucus PC content was demonstrated to be reduced by about 70%, irrespective of the presence of inflammation. This may be of primary pathogenetic significance allowing bacteria to enter the mucus and induce mucosal inflammation. Therefore, a new therapeutic strategy is being developed to substitute the missing mucus PC content in UC. Indeed, a delayed-release PC formulation was able to compensate the lack of PC and improve the inflammatory activity. In randomized controlled studies, delayed-release PC was proven to be clinically and endoscopically effective, which now awaits a phase III authority approval trial.
Oil-gelling properties of soy Lecithin fractions
Food Funct 2021 Nov 1;12(21):10390-10396.34664578 10.1039/d1fo01250a
Lecithin is a mixture of amphiphilic lipids with health benefits. In this study, four different fractions (ethanol soluble, ethanol insoluble, phospholipid and glycolipid fractions) from soy Lecithin were obtained and evaluated as oleogelators. As with the parent Lecithin, the ethanol insoluble fraction (EIF) was unable to function as an oleogelator. The ethanol soluble fraction (ESF) and phospholipid fraction (PLF) formed oleogels at 30% (wt%), while the glycolipid fraction (GLF) formed oleogels at 15%. ESF resulted in an oleogel with a similar appearance and microstructure, but a harder and less cohesive texture than the PLF-supported oleogel. The oleogels formed with GLF were different from those formed with ESF and PLF in appearance and microstructure. GLF at 20% formed an oleogel with better texture characteristics (in the light of hardness) and oil-holding capacity than those formed with 30% of ESF and PLF. This is the first study to investigate the oil-gelling properties of fractions from soy Lecithin. Our results show that the naturally occurring glycolipids from soy Lecithin exhibit great potential as oleogelators.
Lecithin liposomes and microemulsions as new chromatographic phases
J Chromatogr A 2020 Jan 25;1611:460596.31610920 10.1016/j.chroma.2019.460596
Lecithins are phospholipidic mixtures that can be part of microemulsions and liposomes. In this work, ready-to-use preparations of Lecithin have been tested as pseudostationary and mobile phases in EKC and LC, respectively. The selectivity of two EKC systems, one based on Lecithin microemulsions (LMEEKC) and another on liposomes (LLEKC), and of a LC system based on Lecithin microemulsions (MELC) has been evaluated through the solvation parameter model. In all cases, solute volume and hydrogen-bond basicity are the main descriptors that drive the partition process. While solute volume favors the retention of solutes, hydrogen-bond basicity has the contrary effect. In lecithin-based EKC systems the hydrogen-bond acidity of the solute leads to a higher retention while in the lecithin-based LC system a minor retention is produced. The three Lecithin systems have been compared through the solvation parameter model to other chromatographic systems, most of them containing phospholipids. Principal component analysis reveals that Lecithin systems cluster together with the other EKC systems based on phospholipids, with an immobilized artificial membrane (IAM) LC system, with the octanol/water reference partition system, and with a SDS-based microemulsion. Thus, they all show similar selectivity. However, the great advantage of using the ready-to use Lecithin systems is that the laborious liposome preparation is avoided, and that their commercial availability makes them more affordable than IAM LC columns. Finally, taking into account that Lecithin has a high semblance to the mammalian cell membranes composition, the ability of the three Lecithin systems to mimic the pass of the solutes through the membranes has been evaluated. Experimental determinations have demonstrated that the skin partition of neutral solutes can be easily emulated, especially using the lecithin-microemulsion EKC method. The model is robust and shows good prediction ability.
Middle purity soy Lecithin is appropriate for food grade nanoliposome: Preparation, characterization, antioxidant and anti-inflammatory ability
Food Chem 2022 Sep 30;389:132931.35500405 10.1016/j.foodchem.2022.132931
The purity of soy Lecithin exerts significant impact on nanoliposome (NL) properties for food applications. In this study, three soy Lecithin of different purity were used to prepare NL. LC-MS analysis confirmed soy Lecithin of relatively low purify (50% and 70%) contains multiple natural phospholipids. NL produced by soy Lecithin of middle purity (70%) is smaller and more stable than other counterparts. Ultimately, soy Lecithin of 70% purity was selected to develop NL encapsulated crocetin (CR) as model payload and further coated by chitosan (CS). The structure characteristic, physicochemical properties, antioxidant activity and anti-inflammatory activity of crocetin nanoliposome (CR-NL) and chitosan coated crocetin nanoliposome (CS-CR-NL) were evaluated. NL encapsulation and CS coating significantly improve antioxidant and anti-inflammatory ability of CR, and prolong storage period of CR (p < 0.05). For food applications, soy Lecithin of middle purity (70%) is cheaper and more appropriate than soy Lecithin of high purity.