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Laurdan 目录号 GC18338

规格 价格 库存 购买数量
10mg
¥481.00
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25mg
¥1,144.00
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50mg
¥1,924.00
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100mg
¥3,614.00
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Sample solution is provided at 25 µL, 10mM.

质量管理

Quality Control & SDS

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实验参考方法

Samples

bacteria, cells, and liposomes.

Preparation method

1mg/ml in ethanol, 30mg/ml in DMSO & DMF. Stock solution can be stored below -20℃ for several months.

Reaction Conditions

The excitation wavelength is 360 nm, and emission wavelength is 440 nm and 490 nm. [1]

Applications

Measurements of laurdan generalized polarization (GP) Laurdan is an environment-sensitive fluorescent probe, which is conventionally used for monitoring phase state of lipid membranes. [1] For liposomes, using Laurdan-containing liposomes (Laurdan/lipid molar ratio is 1:200), the vesicle suspension was added to the vesicle suspension containing 40 mM KCl, 50 μM EGTA and 10 mM Tris / HCl (pH 7.5) buffer (final lipid concentration is 50μM), and laurdan fluorescence measured before and after adding various experiments.[1] For cell membranes, the erythrocyte plasma membrane was diluted to 3 ml in 0.01 M phosphate saline buffer. Then Laurdan was added to the membrane to a final concentration of 1 μM, and incubated at 37°C for 60 minutes to incorporate Laurdan into the red blood cell membrane. The steady-state fluorescence intensity of Laurdan was measured at 37°C by a spectrofluorometer. The fluorescence measured in the membrane without Laurdan is always subtracted from the data. Use FLWinLab software to normalize the spectrum. [2] Laurdan fluorescence is measured (λex = 360 nm) by a Cary Eclipse spectrofluorimeter. Emission wavelengths, corresponding to the blue and red peaks of laurdan, were 440 and 490 nm. The generalized polarization (GP) was defined as GP = (I440-I490)/ (I440 + I490), where I440 and I490 are the emission intensities at 440 and 490 nm, respectively. GP can theoretically assume values from +1 (being most ordered) and − 1 (being least ordered).[1]

References:

[1]. Mikhail V. Dubinina, Alena A. Semenovaa, Anna I. Ilzorkinaa, Irina B. Mikheevab, Valery A. Yashinc, Nikita V. Penkovc, Valentina A. Vydrinad, Gumer Yu. Ishmuratovd, Vyacheslav A. Sharapova, Ekaterina I. Khoroshavinaa, Sergey V. Gudkove, Konstantin N. Belosludtsev. Effect of betulin and betulonic acid on isolated rat liver mitochondria and liposomes. BBA – Biomembranes, 2020.1862:183383.

[2]. Vignini Arianna, Alia Sonila, Pugnaloni Sofia, Giulietti Alessia, Bacchetti Tiziana, Mazzanti Laura, Luzzi Simona, Fiorini Rosamaria. Erythrocyte membrane fluidity in mild cognitive impairment and Alzheimer's disease patients. Exp Gerontol. 2019.12;128:110754.

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Chemical Properties

Cas No. 74515-25-6 SDF Download SDF
别名 N/A
化学名 1-[6-(dimethylamino)-2-naphthalenyl]-1-dodecanone
Canonical SMILES CN(C)C1=CC=C2C(C=CC(C(CCCCCCCCCCC)=O)=C2)=C1
分子式 C24H35NO 分子量 353.5
溶解度 1mg/ml in ethanol, 30mg/ml in DMSO & DMF 储存条件 Store at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

产品描述

Laurdan is a membrane-permeable fluorescent probe that displays spectral sensitivity to the phospholipid phase of the cell membrane to which it is bound.[1] Quantitation of generalized polarization (GP) of laurdan can be used to identify phospholipid phase. When excited at 340 nm, GP values are 0.6 and -0.2 for gel phase and liquid crystalline phase, respectively. GP does not change with polar head group or pH (in the range 4-10); it changes only with phase state.

Reference:
[1]. Parasassi, T., De Stasio, G., Ravagnan, G., et al. Quantitation of lipid phases in phospholipid vesicles by the generalized polarization of Laurdan fluorescence. Biophys. J. 60(1), 179-189 (1991).