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Cyanine Dyes(花青染料)

Cyanine dyes are molecules containing polymethine bridge between two nitrogen atoms with a delocalized charge.

Due to their structure, cyanines have outstandingly high extinction coefficients often exceeding 100,000 Lmol-1cm-1. Different substituents allow to control properties of the chromophore, such as absorbance wavelength, photostability, and fluorescence. For example, absorbance and fluorescence wavelength can be controlled by a choice of polymethine bridge length: longer cyanines possess higher absorbance and emission wavelengths up to near infrared region.

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Cyanine dyes are molecules containing polymethine bridges between two nitrogen atoms with a delocalizedcyanine_dyes charge which have been used for many years as dyes in life science. Since the Cy3, Cy5 and Cy7 were made commercially available as succinimidyl esters in the early 1990s, cyanine dyes began to be used widely as labels for nucleic acids. Because of the good properties of low non-specific binding to biomolecules and bright fluorescence owing to their huge extinction coefficients and good quantum yields, the Cy dyes have been widespread used in DNA and RNA labelling. Cyanine dyes can be divided into two groups based on water solubility: non-sulfonated cyanines and sulfonated cyanines.

Non-sulfonated cyanines
Available non-sulfonated dyes include Cy3, Cy3.5, Cy5, Cy5.5, Cy7, and Cy7.5. Cy stands for 'cyanine', and the first digit identifies the number of carbon atoms between the indolenine groups. The suffix .5 is added for benzo-fused cyanines. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. They are organic co-solvent soluble (5-20% of DMF or DMSO). When these molecules are used for biomolecule labeling, they should be dissolved in organic solvent first, and added to a solution of biomolecule (protein, peptide, amino-labeled DNA) in appropriate aqueous buffer. Fluorescent properties of non-sulfonated cyanines have little dependence on solvent and surrounding.

Sulfonated cyanines
Available sulfonated cyanines include sulfo-Cy3, sulfo-Cy5, and sulfo-Cy7, which have additional sulfo-groups that facilitate dissolution of dye molecules in aqueous phase. Charged sulfonate groups decrease aggregation of dye molecules and heavily labeled conjugates. Sulfonated cyanines are highly water soluble, and they do not use organic co-solvent for the labeling in aqueous environment.

  1. Cat.No. 产品名称 Information
  2. GC15113 Cy3 alkyne (non-sulfonated) used for Click chemistry to label any molecule bearing azide group.
  3. GC14171 Cy3 azide used for Click chemistry to label alkyne-modified oligonucleotides
  4. GC17605 Cy3 azide (non-sulfonated) used for Click chemistry to label alkyne-modified oligonucleotides
  5. GC19616 Cy3 Bis carboxylic acid 非反应性荧光团,用于对照实验和校准,溶于水
  6. GC19614 Cy3 Bis NHS ester 荧光染料,含有两个氨基反应基团,溶于水
  7. GC17551 Cy3 carboxylic acid (non-sulfonated) Non-reactive fluorophore, for control experiments, and for calibration
  8. GC19618 Cy3 carboxylic acid(Et) 非反应性荧光团,用于对照实验和校准 ,溶于水
  9. GC11356 Cy3 hydrazide (non-sulfonated)

    carbonyl-reactive dye

  10. GC17345 Cy3 NHS ester Cy3 NHS 酯 (Cyanine3 NHS 酯) 是一种黄色荧光染料,用于标记生物分子中的氨基。
  11. GC12618 Cy3 NHS ester (non-sulfonated)

    Protocol for Preparation and purification of Cyanine3 NHS ester -Protein(pro-FD):Dried dyes(Cyanine3 NHS ester) were dissolved to 1 mg/ml in anhydrous DMF and stored frozen in aliquots.

  12. GC19612 Cy3 NHS ester(Et) 氨基反应性荧光染料,溶于水
  13. GC16524 Cy3-dUTP Direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation
  14. GC35767 Cy3-N3 Cy3-N3 是 Cy3 叠氮化物荧光染料,用于多肽、蛋白和寡核苷酸中的氨基。
  15. GC35768 CY3-SE An amine-reactive fluorescent probe
  16. GC30161 CY3-YNE CY3-YNE是一种染料,可用于标记可溶性蛋白、肽和寡核苷酸/DNA。
  17. GC19610 Cy3.5 alkyne (non-sulfonated) 用于点击化学反应的染料炔
  18. GC12923 Cy3.5 azide (non-sulfonated) used for Click chemistry to label alkyne-modified oligonucleotides
  19. GC13938 Cy3.5 hydrazide (non-sulfonated) carbonyl-reactive dye
  20. GC12024 Cy3.5 maleimide (non-sulfonated) Labeling of cysteine residues, as well as other thiolated molecules
  21. GC17971 Cy3.5 NHS ester (non-sulfonated) 标记生物分子中的氨基。
  22. GC35769 CY5 A cyanine-containing fluorochrome
  23. GC47136 Cy5 (triethylamine salt) A cyanine-containing fluorochrome
  24. GC15007 Cy5 alkyne (non-sulfonated) used for Click chemistry to label any molecule bearing azide group.
  25. GC14394 Cy5 amine (non-sulfonated) can be conjugated with reactive groups such as NHS esters, carboxy groups (after carbodiimide activation), and epoxides.
  26. GC12452 Cy5 azide 用于点击化学以标记炔烃修饰的寡核苷酸
  27. GC12480 Cy5 azide (non-sulfonated) used for Click chemistry to label alkyne-modified oligonucleotides
  28. GC19617 Cy5 Bis carboxylic acid 非反应性荧光团,用于对照实验和校准,溶于水
  29. GC19615 Cy5 Bis NHS ester

    荧光染料,含有两个氨基反应基团,溶于水

  30. GC10748 Cy5 Boc-hydrazide (non-sulfonated) carbonyl-reactive dye
  31. GC15339 Cy5 carboxylic acid Non-reactive fluorophore, for control experiments, and for calibration
  32. GC12471 Cy5 carboxylic acid (non-sulfonated) Non-reactive fluorophore, for control experiments, and for calibration
  33. GC19619 Cy5 carboxylic acid(Et) 非反应性荧光团,用于对照实验和校准 ,溶于水
  34. GC15809 Cy5 hydrazide (non-sulfonated) carbonyl-reactive dye
  35. GC18124 Cy5 maleimide (non-sulfonated) Labeling of cysteine residues, as well as other thiolated molecules
  36. GC12907 Cy5 NHS ester

    Cy5 NHS ester(Cyine5-NHS)是一种红色荧光染料,用于标记肽、蛋白质和寡核苷酸中的氨基。

  37. GC10047 Cy5 NHS ester (non-sulfonated) 标记生物分子中的氨基。
  38. GC19613 Cy5 NHS ester(Et) 氨基反应性荧光染料,溶于水
  39. GC30247 CY5-N3 CY5-N3是一种Cy5-叠氮化物,是一种荧光染料。
  40. GC61630 Cy5-PEG3-azide Cy5-PEG3-azide是一种PROTAClinker,属于PEG类。可用于合成PROTAC分子。
  41. GC35771 CY5-SE An amine-reactive fluorescent probe
  42. GC47137 Cy5-SE (triethylamine salt) An amine-reactive fluorescent probe
  43. GC30117 CY5-YNE CY5-YNE是一种用于标记肽,蛋白质,寡核苷酸的氨基基团的反应染料。
  44. GC39818 Cy5.5

    Cy5.5 (Sulfo-Cyanine5.5) 是用于标记生物分子的近红外荧光染料 (Ex=673 nm, Em=707 nm),如肽,蛋白质和寡核苷酸。

  45. GC19611 Cy5.5 alkyne (non-sulfonated) 远红外/近红外染料,用于点击化学标记
  46. GC14684 Cy5.5 azide (non-sulfonated) used for Click chemistry to label alkyne-modified oligonucleotides
  47. GC16132 Cy5.5 carboxylic acid (non-sulfonated) Non-reactive fluorophore, for control experiments, and for calibration
  48. GC12343 Cy5.5 hydrazide (non-sulfonated) carbonyl-reactive dye
  49. GC10412 Cy5.5 maleimide (non-sulfonated) Labeling of cysteine residues, as well as other thiolated molecules
  50. GC11653 Cy5.5 NHS ester (non-sulfonated)

    Cy5.5-NHS酯是一种近红外胺活性染料,用于标记肽、蛋白质和寡核苷酸的氨基。

  51. GC35772 CY7 A cyanine-containing fluorochrome

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