L-Cycloserine ((S)-4-Amino-3-isoxazolidone)
(Synonyms: 环丝氨酸,(S)-Cycloserine; (S)-4-Amino-3-isoxazolidone) 目录号 : GC30862
An inhibitor of sphingolipid synthesis and aminotransferases
Cas No.:339-72-0
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
L-Cycloserine is a potent inhibitor of serine palmitoyl transferase (SPT), the first enzyme in the sphingolipid synthesis pathway.1 It inhibits bacterial SPT activity by 80% at a concentration of 25 ?M, which is 100 times more potent than D-cycloserine . L-Cycloserine inhibits SPT activity up to 86% in a dose-dependent manner in microsomes prepared from mouse brain following administration of doses ranging from 25-200 mg/kg. It also inhibits SPT in rabbit aorta and several aminotransferases in vitro and in vivo in rat.2,3 L-Cycloserine inhibits the growth of M. tuberculosis through branched chain aminotransferase inactivation, and it does so more rapidly and potently than D-cycloserine (MICs = 0.3 and 2.3 ?g/ml, respectively).4
1.Sundaram, K.S., and Lev, M.Inhibition of sphingolipid synthesis by cycloserine in vitro and in vivoJ. Neurochem.42(2)577-581(1984) 2.Williams, R.D., Sgoutas, D.S., Zaatari, G.S., et al.Inhibition of serine palmitoyltransferase activity in rabbit aorta by L-cycloserineJ. Lipid Res.28(12)1478-1481(1987) 3.Wong, D.T., Fuller, R.W., and Molloy, B.B.Inhibition of amino acid transaminases by L-cycloserineAdv. Enzyme Regul.11139-154(1973) 4.Amorim Franco, T.M., Favrot, L., Vergnolle, O., et al.Mechanism-based inhibition of the Mycobacterium tuberculosis branched-chain aminotransferase by D- and I-cycloserineACS Chem Biol.12(5)1235-1244(2017)
| Cas No. | 339-72-0 | SDF | |
| 别名 | 环丝氨酸,(S)-Cycloserine; (S)-4-Amino-3-isoxazolidone | ||
| Canonical SMILES | O=C1NOC[C@@H]1N | ||
| 分子式 | C3H6N2O2 | 分子量 | 102.09 |
| 溶解度 | Water : ≥ 36 mg/mL (352.63 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 9.7953 mL | 48.9764 mL | 97.9528 mL |
| 5 mM | 1.9591 mL | 9.7953 mL | 19.5906 mL |
| 10 mM | 0.9795 mL | 4.8976 mL | 9.7953 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。