IraZolve-Mito
目录号 : GC43908
IraZolve-Mito is a fluorescent probe that can be used to label mitochondria in live cells and tissue sections.
Cas No.:2172800-69-8
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >90.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
a. Reconstitute 0.5 mg IraZolve-Mito with 55 μL DMSO to prepare a 10 mM IraZolve-Mito stock solution. b. Store IraZolve-Mito stock solution at 4°C protected from light. Note 1: IraZolve-Mito stock solution should be used within two months of reconstitution for best staining results.
a. Adherent cells: i．Grow cells in 6-well plate on coverslips to desired confluency (~70-80%). ii. Remove culture medium and add pre-warmed PBS (37°C) or serum-free medium containing IraZolve-Mito at a final concentration of 10-50 μM. iii. Incubate cells at 37°C, 5% CO2 for 30 minutes. iv. Wash cells 2 x 1 minute in PBS. v. Mount coverslips in aqueous mounting media. vi. Observe cells using fluorescence technique of choice. Note 2: Glycerol based mounting media may reduce fluorescence intensity of IraZolve-Mitomm b. Suspension cells: i．Pellet cell suspension and remove supernatant. ii. Resuspend cells in pre-warmed PBS (37°C) or serum-free medium containing IraZolve-Mito at a final concentration of 10-50 μM. iii. Incubate cells at 37°C, 5% CO2 for 30 minutes. iv. Re-pellet the cells and resuspend in PBS or serum-free medium. v. Pipette cells onto a coverslip for imaging in PBS or serum-free medium OR adhere cells to a poly-L-lysine (or similar) coated coverslip by pipetting cells onto the coverslip, allow cells to settle for 2-5 minutes, and wet mount coverslip. vi. Observe using fluorescence technique of choice. Note 3: Optimal staining may vary between cell lines. Staining conditions may be modified according to cell type. Note 4: For epifluorescence applications, IraZolve-Mito can be excited at approximately 365 nm (UV) or405 nm. For confocal and two-photon applications, it can be excited at 400 nm and 800-830 nm, respectively.
**Note 5: Tissue can be stained immediately upon collection or stored for later staining. IraZolve-Mito is compatible with tissue preserved using 4% paraformaldehyde fixation and flash freezing. Note 6: To quench endogenous fluorescence, incubate samples in PBS (pH 7.4) with 100 mM glycine for 20 minutes at room temperature. Other methods to quench fluorescence may be used, such as UV irradiation, however, harsh treatments may induce lipid leaching and/or interfere with lipid binding and should be avoided. a. Staining tissue sections: i. Incubate fresh, fixed, or thawed tissue samples with PBS containing IraZolve-Mito at a final concentration of 10-50μM for 30 minutes at room temperature. ii. Wash samples 3 x 5 minutes in PBS. iii. Mount coverslips using aqueous mounting media. iv. Observe using fluorescence technique of choice. This protocol only provides a guideline, and should be modified according to your specific needs. |
IraZolve-Mito is a fluorescent probe that can be used to label mitochondria in live cells and tissue sections. It can easily penetrate the cell membrane and accumulate in mitochondria, and its fluorescence can be evaluated for mitochondrial structure using fluorescence microscopy, confocal microscopy, and two-photon microscopy. The excitation/emission peaks of IraZolve-Mito are 405/600 nm. It is suitable for live cell and tissue applications.
IraZolve-Mito是一种荧光探针，可用于标记活细胞和组织切片中的线粒体。它能够轻松穿过活细胞膜并富集在线粒体中，其荧光可以通过荧光显微镜、共聚焦显微镜和双光子显微镜来评估线粒体结构。IraZolve-Mito的激发/发射峰值分别为405/600 nm，可用于活细胞和组织应用。
| Cas No. | 2172800-69-8 | SDF | |
| 化学名 | (OC-6-44)-[4-[6-(2-methyl-2H-tetrazol-5-yl-κN4)-3-pyridinyl-κN]benzonitrile]bis[2-(2-pyridinyl-κN)phenyl-κC]-iridium(1+), monohexafluorophosphate(1-) | ||
| Canonical SMILES | CN1N=[N]([Ir+3]23([C-]4=CC=CC=C4C5=CC=CC=[N]25)([C-]6=CC=CC=C6C7=CC=CC=[N]37)[N]8=CC(C9=CC=C(C#N)C=C9)=CC=C%108)C%10=N1.[F-][P+5]([F-])([F-])([F-])([F-])[F-] | ||
| 分子式 | C36H26IrN8 • F6P | 分子量 | 907.8 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.1016 mL | 5.5078 mL | 11.0156 mL |
| 5 mM | 0.2203 mL | 1.1016 mL | 2.2031 mL |
| 10 mM | 0.1102 mL | 0.5508 mL | 1.1016 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Cathodoluminescence imaging of cellular structures labeled with luminescent iridium or rhenium complexes at cryogenic temperatures
Sci Rep 2022 Aug 4;12(1):13432.PMID:35927332DOI:10.1038/s41598-022-17723-w.
We report for the first time the use of two live-cell imaging agents from the group of luminescent transition metal complexes (IraZolve-Mito and REZOLVE-ER) as cathodoluminescent probes. This first experimental demonstration shows the application of both probes for the identification of cellular structures at the nanoscale and near the native state directly in the cryo-scanning electron microscope. This approach can potentially be applied to correlative and multimodal approaches and used to target specific regions within vitrified samples at low electron beam energies.
Mitochondrial imaging in live or fixed tissues using a luminescent iridium complex
Sci Rep 2018 May 29;8(1):8191.PMID:29844412DOI:10.1038/s41598-018-24672-w.
Mitochondrial morphology is important for the function of this critical organelle and, accordingly, altered mitochondrial structure is exhibited in many pathologies. Imaging of mitochondria can therefore provide important information about disease presence and progression. However, mitochondrial imaging is currently limited by the availability of agents that have the capacity to image mitochondrial morphology in both live and fixed samples. This can be particularly problematic in clinical studies or large, multi-centre cohort studies, where tissue archiving by fixation is often more practical. We previously reported the synthesis of an iridium coordination complex [Ir(ppy)2(MeTzPyPhCN)]+; where ppy is a cyclometalated 2-phenylpyridine and TzPyPhCN is the 5-(5-(4-cyanophen-1-yl)pyrid-2-yl)tetrazolate ligand; and showed that this complex (herein referred to as IraZolve-Mito) has a high specificity for mitochondria in live cells. Here we demonstrate that IraZolve-Mito can also effectively stain mitochondria in both live and fixed tissue samples. The staining protocol proposed is versatile, providing a universal procedure for cell biologists and pathologists to visualise mitochondria.