Indigo carmine
(Synonyms: 酸性蓝74,Indigotindisulfonate sodium; C.I.Acid Blue 74) 目录号 : GC39771
An indigoid dye and a redox indicator
Cas No.:860-22-0
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >96.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Indigo carmine is an indigoid dye and a redox indicator.1,2 Upon reduction, indigo carmine shifts from blue to yellow with absorbance maxima of 610 and 407 nm, respectively.2 Formulations containing indigo carmine have been used as color additives in food and pharmaceutical preparations.
1.Claus, H., Faber, G., and K?nig, H.Redox-mediated decolorization of synthetic dyes by fungal laccasesAppl. Microbiol. Biotechnol.59(6)672-678(2002) 2.Tratnyek, P.G., Reilkoff, T.E., Lemon, A.W., et al.Visualizing redox chemistry: Probing environmental oxidation-reduction reactions with indicator dyesChem. Educator6(3)172-179(2001)
| Cas No. | 860-22-0 | SDF | |
| 别名 | 酸性蓝74,Indigotindisulfonate sodium; C.I.Acid Blue 74 | ||
| Canonical SMILES | O=S(C1=CC2=C(N/C(C2=O)=C3NC4=C(C=C(S(=O)(O[Na])=O)C=C4)C\3=O)C=C1)(O[Na])=O | ||
| 分子式 | C16H8N2Na2O8S2 | 分子量 | 466.35 |
| 溶解度 | DMF: slightly soluble,DMSO: 1 mg/ml,PBS (pH 7.2): 10 mg/ml | 储存条件 | Store at -20°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.1443 mL | 10.7216 mL | 21.4431 mL |
| 5 mM | 0.4289 mL | 2.1443 mL | 4.2886 mL |
| 10 mM | 0.2144 mL | 1.0722 mL | 2.1443 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Oral Indigo carmine for the detection of colon adenoma
Scand J Gastroenterol 2021 Mar;56(3):351-355.PMID:33378628DOI:10.1080/00365521.2020.1867897.
Objectives: Colonoscopy is currently considered the optimal method to detect colorectal neoplasia; however, some adenomas remain undetected. While Indigo carmine staining with a dye-spray catheter has demonstrated promising results for reducing the miss rate, we investigated the oral Indigo carmine method. The aim of this study was to determine whether oral Indigo carmine intake before standard colonoscopy increases the adenoma (and adenocarcinoma) detection rate (ADR) or the mean number of adenomas per patient (MAP). Materials and methods: The oral Indigo carmine method was performed from April 2018 to July 2020 in two hospitals. Data were collected in a prospective manner and compared to the conventional group whose data were collected retrospectively and consecutively from January 2016 to March 2018. All data were anonymized. Results: Among the 704 patients included, colonoscopies were completely performed in 693 patients (347 in the indigo group). The ADR did not significantly differ between the groups: 42.3% vs. 40.3% (indigo vs. conventional group; odds ratio: 1.13; 95% confidence interval: 0.9-1.33, p = .33). The MAP was significantly greater in the indigo group (1.15) than that in the conventional group (0.82; p = .009). The cecal intubation rate and time to cecal intubation did not differ between the indigo and conventional group (98.6% vs. 98.3%, p = .83, and 6.2 min vs. 5.9 min, p = .39, respectively). Conclusion: The routine use of oral Indigo carmine does not lead to a higher ADR despite the higher MAP.
Indigo carmine in a Food Dye: Spectroscopic Characterization and Determining Its Micro-Concentration through the Clock Reaction
Molecules 2022 Jul 29;27(15):4853.PMID:35956810DOI:10.3390/molecules27154853.
Indigo carmine is a commonly used industrial blue dye. To determine its concentration in a commercially available food dye composed of a mixture of Indigo carmine and D-glucose, this paper characterizes it through (ATR, KBr) FTIR micro-Raman as well as UV/Vis and clock: Briggs-Rauscher (BR) oscillatory reaction methods. The Indigo carmine was detected in the bulk food dye only by applying micro-Raman spectroscopy, indicating a low percentage of the Indigo carmine present. This research provides an improvement in the deviations from the experimental Raman spectrum as calculated by the B97D/cc-pVTZ level of theory one, resulting in a better geometrical optimization of the Indigo carmine molecule compared to data within the literature. The analytical curves used to determine Indigo carmine concentrations (and quantities) in an aqueous solution of food dye were applied by means of UV/Vis and BR methods. BR yielded significantly better analytical parameters: 100 times lower LOD and LOQ compared to commonly used UV/Vis. The remarkable sensitivity of the BR reaction towards Indigo carmine suggests that not only does Indigo carmine react in an oscillatory reaction but also its decomposition products, meaning that the multiple oxidation reactions have an important role in the BR's Indigo carmine mechanism. The novelty of this research is the investigation of Indigo carmine using a clock BR reaction, opening new possibilities to determine Indigo carmine in other complex samples (pharmaceutical, food, etc.).
Indigo carmine extravasation to upper limb after pelvic reconstructive surgery
Obstet Gynecol 2013 Feb;121(2 Pt 2 Suppl 1):449-51.PMID:23344405DOI:10.1097/aog.0b013e31826d21ec.
Background: The use of dyes during cystoscopy to visualize the ureters adequately is prevalent in gynecologic surgery. Observing ureteral patency after procedures such as a hysterectomy or pelvic reconstruction is important for identifying injury to the upper urinary tract. Indigo carmine is commonly used. Knowing the possible adverse effects and being aware of unusual presentations with the use of Indigo carmine are important in managing and counseling patients. Case: We present a case in which a patient developed Indigo carmine extravasation to her upper limb after pelvic reconstructive surgery. The blue discoloration disappeared after 1 day. Conclusion: Indigo carmine extravasation to other parts of the body can occur without long-term complications. It takes 24-48 hours for the dye to clear subcutaneously.
The Biodegradation of Indigo carmine by Bacillus safensis HL3 Spore and Toxicity Analysis of the Degradation Products
Molecules 2022 Dec 4;27(23):8539.PMID:36500632DOI:10.3390/molecules27238539.
The aims of this article were to investigate Bacillus safensis HL3 spore for its capacity to degrade and detoxify Indigo carmine and to provide an effective biological agent for the treatment of isatin dye wastewater. Bacillus safensis HL3 spore was found to decolorize Indigo carmine by 97% in the presence of acetosyringone within 2 h. Significantly increased activities of spore laccase, intracellular tyrosinase, and lignin peroxidase upon exposure to Indigo carmine were observed. The results of RT-qPCR also showed that the expression of laccase gene was significantly increased. The spore has the ability to degrade Indigo carmine through oxidization. Furthermore, the pathway by which Indigo carmine is degraded was investigated using liquid chromatography-mass spectrometry analysis to identify the biodegradation products. A detailed pathway of Indigo carmine degradation by bacterial spores was proposed for the first time. Toxicity tests indicated that the biodegradation products of Indigo carmine are non-toxic to Nicotiana tabacum seeds and are less hazardous to human erythrocytes than the original dye. Indigo carmine is a typical recalcitrant dye and severely jeopardizes human health. The results demonstrate the utility of the spore from Bacillus safensis HL3 for the degradation of Indigo carmine and simultaneous reduction of its toxicity.
Indigo carmine Hemodynamic Studies to Treat Vasoplegia Induced by Compound 48/80 in a Swine Model of Anaphylaxis
Braz J Cardiovasc Surg 2022 Mar 10;37(1):20-28.PMID:35274519DOI:10.21470/1678-9741-2020-0622.
Introduction: There are many reasons to believe that the nitric oxide/guanosine 3'5' - cyclic monophosphate (or NO/cGMP) pathway on vasoplegic states is underestimated. To study Indigo carmine (IC) as an alternative to methylene blue was the investigation rationale. Methods: The IC (3mg/kg intravenous infusion) study protocol included five experimental groups; 1) Control group - saline was injected at 0 and 10 minutes; 2) IC group - IC was injected at 0 and saline at 10 minutes; 3) compound 48/80 (C48/80) group - C48/80 was injected at 0 minute and saline at 10 minutes; 4) C48/80 + IC group - C48/80 was injected at 0 minute and IC at 10 minutes; and 5) IC + C48/80 group - IC was injected at 0 minute and C48/80 at 10 minutes. The studies were carried out by registering and measuring hemodynamic and blood gasometric parameters, including continuous cardiac output. Results: 1) The effects of the drugs (IC and C48/80) were more evident in the first 20 minutes of recording; 2) hypotensive responses were more pronounced in the C48/80 groups; 3) IC isolated or applied before C48/80 caused transient pulmonary hypertension; and 4) after the first 20 minutes, the pressure responses showed stability with apparent hypotension more pronounced in the C48/80 groups. Clinical observations showed significant hemodynamic instability and catastrophic anaphylactic reactions (agitation, pulmonary hypertension, severe bronchospasm, urticaria, high-intensity cyanosis, violent gastric hypersecretion, and ascites). Conclusion: A global results analysis showed differences between groups only in the first 20 minutes of the experiments.