Hydrocortisone-d4
(Synonyms: 氘代氢化可的松,Cortisol-d4) 目录号 : GC47440An internal standard for the quantification of hydrocortisone
Cas No.:73565-87-4
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Hydrocortisone-d4 is intended for use as an internal standard for the quantification of hydrocortisone/cortisol by GC- or LC-MS. Cortisol, known as hydrocortisone when used as a therapeutic, is a glucocorticoid produced by the adrenal cortex in response to adrenocorticotropic hormone (ACTH).1,2 It is an agonist at the mineralocorticoid receptor (MR) and the glucocorticoid receptor (GR), with an approximately 6- to 10-fold greater affinity for MR. Cortisol production is increased during periods of stress, and it is a major effector molecule in the hypothalamic-pituitary-adrenal axis (HPA) stress response.2 Cortisol levels increase with age and are often elevated in major depressive disorder, certain forms of hypertension, and Parkinson's disease.3,4,5
1.Krieger, D.T.Rhythms of ACTH and corticosteroid secretion in health and disease, and their experimental modificationJ. Steroid Biochem.6(5)785-791(1975) 2.Dunlop, B.W., and Wong, A.The hypothalamic-pituitary-adrenal axis in PTSD: Pathophysiology and treatment interventionsProg. Neuropsychopharmacol. Biol. Psychiatry89361-379(2019) 3.Quinkler, M., and Stewart, P.M.Hypertension and the cortisol-cortisone shuttleJ. Clin. Endocrinol. Metab.88(6)2384-2392(2003) 4.Varghese, F.P., and Brown, E.S.The hypothalamic-pituitary-adrenal axis in major depressive disorder: A brief primer for primary care physiciansPrim. Care Companion J. Clin. Psychiatry3(4)151-155(2001) 5.Soares, N.M., Pereira, G.M., Altmann, V., et al.Cortisol levels, motor, cognitive and behavioral symptoms in Parkinson's disease: A systematic reviewJ. Neural Transm. (Vienna)(2018)
| Cas No. | 73565-87-4 | SDF | |
| 别名 | 氘代氢化可的松,Cortisol-d4 | ||
| Canonical SMILES | O=C1CC[C@@]2(C)C(CC[C@]3([H])[C@]2([2H])[C@](O)([2H])C([2H])([2H])[C@@]4(C)[C@@]3([H])CC[C@]4(O)C(CO)=O)=C1 | ||
| 分子式 | C21H26D4O5 | 分子量 | 366.5 |
| 溶解度 | DMF: 30 mg/ml,DMF:PBS (pH 7.2) (1:4): 0.2 mg/ml,DMSO: 20 mg/ml,Ethanol: 2 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.7285 mL | 13.6426 mL | 27.2851 mL |
| 5 mM | 0.5457 mL | 2.7285 mL | 5.457 mL |
| 10 mM | 0.2729 mL | 1.3643 mL | 2.7285 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Evaluation of the diagnostic criteria for Cushing's disease in Japan
Endocr J 2013;60(2):127-35.PMID:23171704DOI:10.1507/endocrj.ej12-0299
Adrenocorticotropic hormone (ACTH)-dependent Cushing's syndrome is caused by an ACTH-producing tumor, as is the case with Cushing's disease and ectopic ACTH syndrome (EAS). Diagnosis and differential diagnosis of Cushing's disease from EAS in ACTH-dependent Cushing's syndrome are thus challenging problems in clinical endocrinology. The diagnostic criteria for Cushing's disease in Japan, established by the working group of the Japan Ministry of Health, Labour and Welfare, were originally reported in 2003 and revised in 2007 and 2010. In addition, criteria for subclinical Cushing's disease were established in Japan in 2010. In this review, we evaluate the usefulness and accuracy of the most recent diagnostic criteria. Previous data suggest that as an initial test of Cushing's syndrome, 0.5 mg dexamethasone is more sensitive than 1 mg in the overnight dexamethasone suppression test (DST). Here, we recommend 0.5 mg plus a plasma cortisol cut-off level of 3 µg/dL as a suitable low-dose overnight DST for screening of all cases of ACTH-dependent Cushing's syndrome in Japan. Recently, standardization of cortisol measurements by the ID-LC/MS/MS method using seven assay kits with standard plasma material containing synthetic Hydrocortisone-d4 was carried out in Japan. The resulting relative standard deviation was within 10%. The cut-off value remains valid even after standardization of plasma cortisol measurements. Although the recent diagnostic criteria achieve higher diagnostic specificity, care should be taken since data for Cushing's disease partially overlaps with some cases of EAS. Overall, therefore, this review suggests that the accuracy of each diagnostic test should be considered.