β-Glycerophosphate (sodium salt hydrate)
(Synonyms: 五水β-甘油磷酸钠(二钠五水合物)) 目录号 : GC11806
An inhibitor of alkaline phosphatase
Cas No.:13408-09-8
Sample solution is provided at 25 µL, 10mM.
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- Purity: >98.00%
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β-Glycerophosphate (sodium salt hydrate) is a potent protein phosphatase inhibitor [1].
Protein phosphatase is an enzyme that removes a phosphate group from the phosphorylated amino acid residue of its target protein. Protein phosphorylation is one of the most common protein posttranslational modifications.
β-Glycerophosphate is a potent protein phosphatase inhibitor that acts as a phosphate group donor in bone cell mineralization studies [1][3]. In MC3T3-E1, ROS 17/2.8, and chick osteoblast-like cells, β-Glycerophosphate did not affect the rate of anaerobic glycolysis, but increased the medium Pi levels, suggesting that β-Glycerophosphate was hydrolyzed by bone cells. The local increase in medium Pi concentration promoted rapid mineral deposition [1]. In human bone marrow stromal cells (HBMSC) and human osteoblasts (HOB), BMP-2 resulted in mineralization of their matrix in the presence of beta-glycerophosphate and ascorbic acid [3]. β-Glycerophosphate (10 mM) also accelerated calcification of cultured vascular smooth muscle cells through an alkaline phosphatase-related mechanism [4].
β-甘油磷酸钠盐水合物是一种强效的蛋白磷酸酶抑制剂[1]。
蛋白磷酸酶是一种酶，可以从其目标蛋白的磷酸化氨基酸残基上移除一个磷酸基团。蛋白质磷酸化是最常见的蛋白质翻译后修饰之一。
β-甘油磷酸钠盐水合物是一种强效的蛋白磷酸酶抑制剂，在骨细胞矿化研究中作为磷酸基团供体[1][3]。在MC3T3-E1、ROS 17/2.8和鸡成骨样细胞中，β-甘油磷酸钠盐水合物不影响无氧糖酵解速率，但会增加培养基中的磷酸盐含量，这表明β-甘油磷酸钠盐水合物被骨细胞水解。培养基中局部磷酸盐浓度的升高促进了快速的矿物沉积[1]。在人类骨髓基质细胞（HBMSC）和人类成骨细胞（HOB）中，β-甘油磷酸钠盐水合物和抗坏血酸存在下，BMP-2促进了它们的基质矿化[3]。β-甘油磷酸钠盐水合物（10 mM）还通过碱性磷酸酶相关机制加速了培养的血管平滑肌细胞的钙化[4]。
References:
[1]. Chung CH, Golub EE, Forbes E, et al. Mechanism of action of beta-glycerophosphate on bone cell mineralization. Calcif Tissue Int. 1992 Oct;51(4):305-11.
[2]. Fujimoto H, Mabuchi I. Isolation of cleavage furrows from eggs of regular sea urchins and identification of furrow-specific proteins. J Biochem. 1997 Sep;122(3):518-24.
[3]. Lecanda F, Avioli LV, Cheng SL. Regulation of bone matrix protein expression and induction of differentiation of human osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2. J Cell Biochem. 1997 Dec 1;67(3):386-96.
[4]. Shioi A, Nishizawa Y, Jono S, et al. Beta-glycerophosphate accelerates calcification in cultured bovine vascular smooth muscle cells. Arterioscler Thromb Vasc Biol. 1995 Nov;15(11):2003-9.
| Cas No. | 13408-09-8 | SDF | |
| 别名 | 五水β-甘油磷酸钠(二钠五水合物) | ||
| 化学名 | 2-(dihydrogen phosphate)-1,2,3-propanetriol, disodium salt, pentahydrate | ||
| Canonical SMILES | [O-]P(OC(CO)CO)([O-])=O.[Na+].[Na+].O.O.O.O.O | ||
| 分子式 | C3H7O6P • 2Na [5H2O] | 分子量 | 306.1 |
| 溶解度 | ≥ 30.7mg/mL in Water, ≥ 14.3mg/mL in insoluble in DMSO | 储存条件 | 4°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.2669 mL | 16.3345 mL | 32.6691 mL |
| 5 mM | 0.6534 mL | 3.2669 mL | 6.5338 mL |
| 10 mM | 0.3267 mL | 1.6335 mL | 3.2669 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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