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Geneticin, G-418 Sulfate 目录号 GC17427

Aminoglycosidic antibiotic

规格 价格 库存 购买数量
1g
¥546.00
现货
5g
¥1,575.00
现货

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Sample solution is provided at 25 µL, 10mM.

质量管理

Quality Control & SDS

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实验参考方法

Cell experiment [1, 2]:

Cell lines

DENV-2-infected BHK cells

Preparation method

This compound is limited soluble in DMSO. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

1–300 μg/ml, 120 h

Applications

Geneticin (1–300 μg/ml) inhibited many pro- and eukaryotes. Geneticin reduced viability of BHK cells with a CC50 value of 165 ± 5 μg/ml. Geneticin prevented DENV-induced CPE and increased cell viability of infected cells with an EC50 value of ~3 ± 0.4 μg/ml. Geneticin inhibited the yield of viral titers of DENV-2 even after 72 hpi with an EC50 value of 2 ± 0.1 μg/ml and an EC90 value of about 20 ± 2 μg/ml. Geneticin inhibited the number of plaques produced by the virus, and significantly reduced the sizes of the remaining plaques.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1] Bar-Nun S, Shneyour Y, Beckmann JS. G-418, an elongation inhibitor of 80 S ribosomes. Biochim Biophys Acta. 1983, 741 (1): 123-7.

[2] Julian Davies and Antonio Jimenez. A new selective agent for eukaryotic cloning vectors. Am. J. Trop. Med. Hyg. 1980, 29 (5): 1089-1092. [3] Xianchao G. Zhang, Peter W. Mason, Edward J. Dubovi, Xiaodong Xu, Nigel Bourne, Randall W. Renshaw, Timothy M. Block, and Alexander V. Birk. Antiviral activity of Geneticin against DENGUE virus. Antiviral Res. 2009 July, 83(1): 21–27.

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Chemical Properties

Cas No. 108321-42-2 SDF
别名 Antibiotic G418
化学名 2-[4,6-diamino-3-[3-amino-4,5-dihydroxy-6-(1-hydroxyethyl)oxan-2-yl]oxy-2-hydroxycyclohexyl]oxy-5-methyl-4-(methylamino)oxane-3,5-diol;sulfuric acid
Canonical SMILES CC(C1C(C(C(C(O1)OC2C(CC(C(C2O)OC3C(C(C(CO3)(C)O)NC)O)N)N)N)O)O)O.OS(=O)(=O)O.OS(=O)(=O)O
分子式 C20H40N4O10.2H2SO4 分子量 692.71
溶解度 <1.34mg/mL in DMSO; ≥240.4 mg/ml in H2O 储存条件 Store at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

产品描述

G-418 is an elongation inhibitor of 80 S ribosomes [1].

G418 is an aminoglycoside antibiotic, it has a wide spectrum of activity and is active against bacteria, yeasts, fungi, algae, plant and even animal cells. G418 is usually used as a cloning selection in pro- and eukaryotes at concentrations from 1 to 300μg/ml. In the studies of antibiotic resistance in clinical isolates of bactenia, it is found that all strains carrying 3'-O-aminoglycoside phosphotransferases are resistant to G418. Among these phosphotransferases, type I and type II are encoded by the transposable elements TnS and Tn601, respectively [2].

Apart from the selector function, G418 is also found to have antiviral activity against bovine viral diarrhea virus (BVDV). G418 prevents the cytopathic effect (CPE) resulting from DENV-2 infection of BHK cells, in a dose-dependent manner with an EC50 value of 3±0.4μg/ml [3].

References:
[1] Bar-Nun S, Shneyour Y, Beckmann JS. G-418, an elongation inhibitor of 80 S ribosomes. Biochim Biophys Acta. 1983, 741 (1): 123-7.
[2] Julian Davies and Antonio Jimenez. A new selective agent for eukaryotic cloning vectors. Am. J. Trop. Med. Hyg. 1980, 29 (5): 1089-1092.
[3] Xianchao G. Zhang, Peter W. Mason, Edward J. Dubovi, Xiaodong Xu, Nigel Bourne, Randall W. Renshaw, Timothy M. Block, and Alexander V. Birk. Antiviral activity of Geneticin against DENGUE virus. Antiviral Res. 2009 July, 83(1): 21–27.