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Hederasaponin B Sale

(Synonyms: 刺五加叶中) 目录号 : GC38050

A triterpene saponin with antiviral and antioxidant activities

Hederasaponin B Chemical Structure

Cas No.:36284-77-2

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1mg
¥702.00
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5mg
¥2,115.00
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10mg
¥3,600.00
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产品描述

Hederasaponin B is a triterpene saponin that has been found in H. helix and has antiviral and antioxidant activities.1,2 It inhibits the cytopathic effect induced by the enterovirus 71 (EV71) genotypes C3 and C4a (EC50s = 24.77 and 41.77 ?g/ml, respectively), as well as reduces expression of the viral structural capsid protein VP2, in Vero cells.1 Hederasaponin B (100, 150, and 200 ?g/ml) inhibits superoxide generation in human neutrophils.2

1.Song, J., Yeo, S.-G., Hong, E.-H., et al.Antiviral activity of hederasaponin B from Hedera helix against enterovirus 71 subgenotypes C3 and C4aBiomol. Ther. (Seoul)22(1)41-46(2014) 2.Chen, X., Lu, J., He, W., et al.Antiperoxidation activity of triterpenoids from rhizome of Anemone raddeanaFitoterapia80(2)105-111(2009)

Chemical Properties

Cas No. 36284-77-2 SDF
别名 刺五加叶中
分子式 C59H96O25 分子量 1205.38
溶解度 DMF: 30 mg/ml,DMSO: 30 mg/ml,PBS (pH 7.2): 5 mg/ml 储存条件 Store at -20°C,protect from light
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1 mg 5 mg 10 mg
1 mM 0.8296 mL 4.1481 mL 8.2961 mL
5 mM 0.1659 mL 0.8296 mL 1.6592 mL
10 mM 0.083 mL 0.4148 mL 0.8296 mL
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Research Update

Structural characterization of the metabolites of orally ingested Hederasaponin B, a natural saponin that is isolated from Acanthopanax senticosus leaves by liquid chromatography-mass spectrometry

J Pharm Biomed Anal 2021 Apr 15;197:113929.PMID:33618133DOI:10.1016/j.jpba.2021.113929.

Plant saponins are important natural product with biologically active. However, the metabolism of these compounds has rarely been studied due to their low bioavailability and the complexity of their metabolite structures. In this study, ultra-performance liquid chromatography/Fusion Lumos Orbitrap mass spectrometry was used to analyze the metabolites of Hederasaponin B in vivo, and its possible metabolic pathways were proposed. After oral administration of the parent drug, a total of 47 metabolites are identified in rat feces (42), urine (11), and plasma (9) samples. These metabolites resulted from the metabolic processes in phases I and II reactions involved in deglycosylation, hydroxylation, acetylation, oxidation, gluconalciation and glycosylations. Deglycosylation is the main metabolic pathway (accounts for 52.46 % of all metabolites in feces samples). Among the identified metabolites, four were glycosylated (deprotonated precursors at m/z = 1335.7, 1365.7, 1467.9, and 1379.6) with higher molecular weight than the parent drug . These glycosylated compounds account for 11.55 % of the metabolites in rat feces according to the semi-quantitative chromatographic peak areas. To sum up, the results of this study provide a basis for further understanding the metabolism of plant saponins in vivo.

Antiviral Activity of Hederasaponin B from Hedera helix against Enterovirus 71 Subgenotypes C3 and C4a

Biomol Ther (Seoul) 2014 Jan;22(1):41-6.PMID:24596620DOI:10.4062/biomolther.2013.108.

Enterovirus 71 (EV71) is the predominant cause of hand, foot and mouth disease (HFMD). The antiviral activity of Hederasaponin B from Hedera helix against EV71 subgenotypes C3 and C4a was evaluated in vero cells. In the current study, the antiviral activity of Hederasaponin B against EV71 C3 and C4a was determined by cytopathic effect (CPE) reduction method and western blot assay. Our results demonstrated that Hederasaponin B and 30% ethanol extract of Hedera helix containing Hederasaponin B showed significant antiviral activity against EV71 subgenotypes C3 and C4a by reducing the formation of a visible CPE. Hederasaponin B also inhibited the viral VP2 protein expression, suggesting the inhibition of viral capsid protein synthesis.These results suggest that Hederasaponin B and Hedera helix extract containing Hederasaponin B can be novel drug candidates with broad-spectrum antiviral activity against various subgenotypes of EV71.

A rapid and sensitive liquid chromatography-tandem mass spectrometric method for the determination of Hederasaponin B in rat plasma: Application to a pharmacokinetic study

Asian J Pharm Sci 2017 Jul;12(4):363-369.PMID:32104347DOI:10.1016/j.ajps.2016.09.001.

A rapid, simple and sensitive ultra-high performance liquid chromatography-tandem mass spectrometric (UPLC-MS/MS) method was developed and validated for the determination of Hederasaponin B, an active triterpenoid saponin widely existed in Hedera helix L. Plasma samples were processed by protein precipitation with acetonitrile and separated on a Thermo Hypersil GOLD C18 (2.1 mm × 50 mm,1.9 µm) at flow rate of 0.3 ml/min, with a gradient elution consisting of acetonitrile and water containing 0.1% (v/v) formic acid at 30 °C and detected by electrospray ionization mass spectrometry in the positive multiple reaction monitoring (MRM) mode. The linearity was found to be within the concentration range of 0.5-5000 ng/ml with a lower limit of quantification of 0.5 ng/ml. The absolute oral bioavailability of Hederasaponin B was 0.24 ± 0.49%. This indicated that the concentration-time course of the Hederasaponin B existed a double-peak phenomenon. This method was further applied to the determination of Hederasaponin B in rat plasma and showed good practicability, for the first time, after intragastric (25 mg/kg) and intravenous (2 mg/kg) administration in rats.

Determination of saponins and flavonoids in ivy leaf extracts using HPLC-DAD

J Chromatogr Sci 2015 Apr;53(4):478-83.PMID:24981979DOI:10.1093/chromsci/bmu068.

A new method for the determination of six compounds, chlorogenic acid, rutin, nicotiflorin, hederacoside C, Hederasaponin B and α-hederin, in ivy leaf extracts using high-performance liquid chromatography with diode array detector was developed. The chromatographic separation was performed on a YMC Hydrosphere C18 analytical column using a gradient elution of 0.1% phosphoric acid and acetonitrile. The method was validated in terms of specificity, linearity (r(2) > 0.9999), precision [relative standard deviation (RSD) < 0.36%] and accuracy (97.4-103.8%). The limit of detection and limit of quantification were <20.32 and 61.56 ng for all analytes, respectively. The tested compounds were found to be stable in the ivy leaf extract from 0 to 48 h, and the RSD value for each compound was <0.90%. The validated method was successfully applied to quantify all six compounds in a 30% ethanol ivy leaf extract and 13 ivy leaf extract products. The results showed that all the tested products satisfied the minimum requirement for the content of hederacoside C. However, there were some differences between the contents of other constituents.

Antiperoxidation activity of triterpenoids from rhizome of Anemone raddeana

Fitoterapia 2009 Mar;80(2):105-11.PMID:19084054DOI:10.1016/j.fitote.2008.11.003.

Four triterpenoid compounds hederacolchiside E (1), Hederasaponin B (2), raddeanoside 20 (3) and raddeanoside 21 (4) were isolated from ethanol extracts of rhizome of Anemone raddeana Regel. The effects of these triterpenoids on superoxide generation, tyrosyl phosphorylation of proteins and translocation of cytosolic compounds, such as p47(phox), p67(phox) and Rac to the cell membrane in human neutrophils was investigated. The superoxide generation induced by N-formyl-methionyl-leucyl-phenylalanine (fMLP) was slightly suppressed by Hederasaponin B, raddeanoside 20 and raddeanoside 21 in a concentration dependent manner. The superoxide generation induced by arachidonic acid (AA) was suppressed by Hederasaponin B and raddeanoside 21 significantly. fMLP- and AA-induced tyrosyl phosphorylation and translocation of the cytosolic proteins: p47(phox), p67(phox), and Rac to the cell membrane were suppressed in parallel with the suppression of stimulus-induced superoxide generation.