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Mc-MMAE Sale

(Synonyms: 马来酰亚胺基己酰-单甲基澳瑞他汀E,Maleimidocaproyl-monomethylauristatin E) 目录号 : GC36561

Mc-MMAE 是保护基团 (马来酰亚氨基己酰) 共轭连接到 MMAE。Mc-MMAE 是有效的微管蛋白抑制剂,可用于偶联抗体。

Mc-MMAE Chemical Structure

Cas No.:863971-24-8

规格 价格 库存 购买数量
10mM (in 1mL DMSO)
¥12,584.00
现货
1mg
¥4,604.00
现货
5mg
¥8,203.00
现货
10mg
¥12,555.00
现货
50mg 待询 待询
100mg 待询 待询

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Sample solution is provided at 25 µL, 10mM.

产品文档

Quality Control & SDS

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实验参考方法

Kinase experiment:

Horseradish peroxidase (HRP) is thiolated with 2-iminothiolane and conjugated to mc-MMAE to generate the HRP-MMAE reporter enzyme-drug conjugate. Briefly, a thiolation reaction mixture containing 0.2 mM HRP (8 mg/mL) and 50 mM 2-iminothiolane in 25 mM sodium borate decahydrate (Na2B4O7•10H2O) buffer (pH 9.0) is incubated for 1 hour at 37°C. Unreacted 2-iminothiolane is removed by passage through a PD-10 desalting column equilibrated in PBS (pH 7.4). Peak fractions are pooled and mc-MMAE is coupled to thiolated HRP (HRP-SH) at a molar ratio of 3:1. The final conjugation reaction mixture contained 80 μM HRP-SH (3.2 mg/mL) in sodium borate buffer [50 mM H3BO3, 50 mM NaCl (pH 8.0); 80% v/v] and 240 μM mc-MMAE in ice-cold CH3CN (20% v/v). After 30 minutes on ice, the reaction is terminated with a 20-fold molar excess of free cysteine (4.8 mM) before PD-10 chromatography. Peak fractions containing HRP-MMAE (exchanged into PBS) are pooled and evaluated for extent of modification using the thiol-reactive dye, Alexa Fluor 594 C5 maleimide[1].

References:

[1]. Sanderson RJ, et al. In vivo drug-linker stability of an anti-CD30 dipeptide-linked auristatin immunoconjugate. Clin Cancer Res. 2005 Jan 15;11(2 Pt 1):843-52.

产品描述

Mc-MMAE is a protective group (maleimidocaproyl)-conjugated monomethyl auristatin E (MMAE), which is a potent tubulin inhibitor. Mc-MMAE is a drug-linker conjugate for ADC. Tubulin, Antibody-drug conjugates (ADC)[1]

Synthesis of maleimidocaproyl-MMAE (mc-MMAE) requires the addition of maleimidocaproic acid to a solution of MMAE in dichloromethane followed by the addition of diethyl cyanophosphonate and diisopropylethylamine[1].

[1]. Sanderson RJ, et al. In vivo drug-linker stability of an anti-CD30 dipeptide-linked auristatin immunoconjugate. Clin Cancer Res. 2005 Jan 15;11(2 Pt 1):843-52.

Chemical Properties

Cas No. 863971-24-8 SDF
别名 马来酰亚胺基己酰-单甲基澳瑞他汀E,Maleimidocaproyl-monomethylauristatin E
Canonical SMILES O=C1N(CCCCCC(N(C)[C@H](C(N[C@@H](C(C)C)C(N([C@@H]([C@@H](C)CC)[C@@H](CC(N2[C@H]([C@H](OC)[C@H](C(N[C@@H]([C@H](C3=CC=CC=C3)O)C)=O)C)CCC2)=O)OC)C)=O)=O)C(C)C)=O)C(C=C1)=O
分子式 C49H78N6O10 分子量 911.18
溶解度 DMSO: ≥ 51 mg/mL (55.97 mM) 储存条件 Store at -20°C,unstable in solution, ready to use.
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储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
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溶解性数据

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1 mg 5 mg 10 mg
1 mM 1.0975 mL 5.4874 mL 10.9748 mL
5 mM 0.2195 mL 1.0975 mL 2.195 mL
10 mM 0.1097 mL 0.5487 mL 1.0975 mL
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