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Fura-FF (potassium salt)

(Synonyms: 钙离子荧光探针FURA-FF,五钾盐) 目录号 : GC43713

A low affinity fluorescent calcium indicator

Fura-FF (potassium salt) Chemical Structure

Cas No.:192140-58-2

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50μg
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产品描述

Fura-FF is a difluorinated derivative of the calcium indicator fura-2. Unlike, fura-2, fura-FF has negligible magnesium sensitivity, thus reducing interference from this cation.[1] Fura-FF also has a higher calcium dissociation constant than fura-2 (Kd(calcium) = 6 and 0.14 µM, respectively).[1],[2]However, the spectral properties of fura-FF and fura-2 are similar with fura-FF displaying excitation/emission spectra of 365/514 nm in the absence of calcium, with a shift to 339/507 nm in the presence of a high calcium concentration.[3] Low affinity calcium dyes, including fura-FF, are preferred for studying compartments with high concentrations of calcium, such as mitochondria, or in cell systems that have relatively low calcium buffering capacities, such as neuronal dendrites and spines.[4],[5[,[6]

Refererence:
[1]. Hyrc, K.L., Bownik, J.M., and Goldberg, M.P. Ionic selectivity of low-affinity ratiometric calcium indicators: mag-Fura-2, Fura-2FF and BTC. Cell Calcium 27(2), 75-86 (2000).
[2]. Grynkiewicz, G., Poenie, M., and Tsien, R.Y. A new generation of Ca2+ indicators with greatly improved fluorescence properties. J. Biol. Chem. 260(6), 3440-3450 (1985).
[3]. Ruggiu, A.A., Bannwarth, M., and Johnsson, K. Fura-2FF-based calcium indicator for protein labeling. Org. Biomol. Chem. 8(15), 3398-3401 (2010).
[4]. Aponte, Y., Bischofberger, J., and Jonas, P. Efficient Ca2+ buffering in fast-spiking basket cells of rat hippocampus. J.Physiol. 586(8), 2061-2075 (2008).
[5]. Canepari, M., Vogt, K., and Zecevic, D. Combining voltage and calcium imaging from neuronal dendrites. Cell.Mol.Neurobiol. 28(8), 1079-1093 (2008).
[6]. Marcu, R., Neely, C.K., Karamanlidis, G., et al. Multi-parameter measurement of the permeability transition pore opening in isolated mouse heart mitochondria. J.Vis.Exp. 67, (2012).

Chemical Properties

Cas No. 192140-58-2 SDF
别名 钙离子荧光探针FURA-FF,五钾盐
化学名 2-[6-[bis(carboxymethyl)amino]-5-[2-[6-[bis(carboxymethyl)amino]-2,3-difluorophenoxy]ethoxy]-2-benzofuranyl]-5-oxazolecarboxylic acid, pentapotassium salt
Canonical SMILES [O-]C(CN(C1=CC2=C(C=C(C3=NC=C(C([O-])=O)O3)O2)C=C1OCCOC(C(F)=C(F)C=C4)=C4N(CC([O-])=O)CC([O-])=O)CC([O-])=O)=O.[K+].[K+].[K+].[K+].[K+]
分子式 C28H18F2N3O14 • 5K 分子量 853.9
溶解度 Soluble in water 储存条件 Store at -20°C
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1 mM 1.1711 mL 5.8555 mL 11.711 mL
5 mM 0.2342 mL 1.1711 mL 2.3422 mL
10 mM 0.1171 mL 0.5855 mL 1.1711 mL
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