FSL-1
目录号 : GC18761
FSL-1是一种合成的二酰基脂肽TLR2/6激动剂。
Cas No.:322455-70-9
Sample solution is provided at 25 µL, 10mM.
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FSL-1 is a synthetic diacyl lipopeptide TLR2/6 agonist [1]. FSL-1 binds to and activates TLR2/6 heterodimers, triggering downstream MyD88-dependent signaling pathways, leading to activation of NF-κB, MAPK, and other pathways, and inducing the release of multiple inflammatory factors (TNF-α, IL-1β, IL-6, IL-8) and chemokines [2-3]. FSL-1 is primarily used in immunology and inflammation research [4].
In THP-1 cells, FSL-1 (50ng/mL; 24h) induces MMP-9 gene expression [5]. In HaCaT cells, FSL-1 (100ng/mL; 0.5-2h) stimulation downregulates TGB6 expression [6].
In radiation mice model, prophylactic administration of FSL-1 (0.25mg/kg; sc; 40d) significantly improves the survival rate of male and female C57BL/6 mice [7]. In radiation mice model, FSL-1 (0.09mg/kg; sc; 8d) stimulates the recruitment of hematopoietic progenitor cells [8].
References:
[1]. Rose W A, McGowin C L, Pyles R B. FSL-1, a bacterial-derived toll-like receptor 2/6 agonist, enhances resistance to experimental HSV-2 infection[J]. Virology journal, 2009, 6(1): 195.
[2]. Kurkjian C J, Guo H, Montgomery N D, et al. The toll–like Receptor 2/6 agonist, FSL–1 lipopeptide, therapeutically mitigates acute radiation syndrome[J]. Scientific Reports, 2017, 7(1): 17355.
[3]. Nakamura J, Shibata K, Hasebe A, et al. Signaling pathways induced by lipoproteins derived from Mycoplasma salivarium and a synthetic lipopeptide (FSL‐1) in normal human gingival fibroblasts[J]. Microbiology and immunology, 2002, 46(3): 151-158.
[4]. Lim R, Barker G, Lappas M. The TLR 2 Ligand FSL‐1 and the TLR 5 Ligand Flagellin Mediate Pro‐Inflammatory and Pro‐Labour Response via MyD88/TRAF 6/NF‐κB‐Dependent Signalling[J]. American Journal of Reproductive Immunology, 2014, 71(5): 401-417.
[5]. Ahmad R, Shihab P K, Jasem S, et al. FSL-1 induces MMP-9 production through TLR-2 and NF-κB/AP-1 signaling pathways in monocytic THP-1 cells[J]. Cellular Physiology and Biochemistry, 2014, 34(3): 929-942.
[6]. Xu M, Huang J, Zhu F, et al. FOXO1 inhibits FSL-1 regulation of integrin β6 by blocking STAT3 binding to the integrin β6 gene promoter[J]. Frontiers in Cellular and Infection Microbiology, 2022, 12: 998693.
[7]. Holmes-Hampton G P, Kumar V P, Valenzia K, et al. FSL-1: A Synthetic Peptide Increases Survival in a Murine Model of Hematopoietic Acute Radiation Syndrome[J]. Radiation Research, 2024, 201(5): 449-459.
[8]. Brickey W J, Caudell D L, Macintyre A N, et al. The TLR2/TLR6 ligand FSL-1 mitigates radiation-induced hematopoietic injury in mice and nonhuman primates[J]. Proceedings of the National Academy of Sciences, 2023, 120(50): e2122178120.
FSL-1是一种合成的二酰基脂肽TLR2/6激动剂 [1]。FSL-1结合并激活TLR2/6异二聚体，触发下游MyD88依赖性信号通路，进而激活NF-κB、MAPK等通路，诱导多种炎症因子（TNF-α、IL-1β、IL-6、IL-8）和趋化因子的释放 [2-3]。FSL-1主要用于免疫学和炎症研究 [4]。
在THP-1细胞中，FSL-1（50ng/mL；24h）可诱导MMP-9基因表达 [5]。在HaCaT细胞中，FSL-1（100ng/mL；0.5-2h）刺激可下调TGB6表达 [6]。
在辐射小鼠模型中，预防性给予FSL-1（0.25mg/kg；sc；40d）显著提高了雄性和雌性C57BL/6小鼠的存活率 [7]。在辐射小鼠模型中，FSL-1（0.09mg/kg；sc；8d）刺激造血祖细胞的募集 [8]。
| Cell experiment [1]: | |
Cell lines | THP-1 cells |
Preparation Method | THP-1 cells were seeded in 12-well plates at 1 × 106 cells per well. Cells were stimulated with FSL-1 (50ng/mL) or TNF-α (25ng/mL) at 37℃ for 24 hours. Cells were harvested for RNA isolation, and conditioned medium was collected for determination of MMP-9 secretion and SEAP activity. Conditioned medium was collected and stored at −80℃. |
Reaction Conditions | 50ng/mL; 24h |
Applications | In THP-1 cells, FSL-1 induces MMP-9 gene expression. |
| Animal experiment [2]: | |
Animal models | Radiation mice model |
Preparation Method | To investigate the radioprotective efficacy of FSL-1, all mice in the study were weighed before the start of the study; animals weighing ± 10% of the mean body weight were excluded. Mice were then randomly divided into two groups (vehicle control and FSL-1 groups), with five animals per cage. Twenty-four hours before radiation exposure, mice received a single dose of 0.25mg/kg of FSL-1 or vehicle (sterile PBS) subcutaneously injected into the scruff of the neck using a 23-G needle. Each drug- and vehicle-treated (PBS) group contained 25 animals. Males received a radiation dose of 8.1Gy. Because females are more radioresistant than males, females received a higher dose of 8.7Gy. Survival was monitored up to four times daily for 30 days, and surviving animals were euthanized upon completion of the study. |
Dosage form | 0.25mg/kg; sc; 40d |
Applications | Prophylactic administration of FSL-1 significantly improves the survival rate of male and female C57BL/6 mice. |
References: | |
| Cas No. | 322455-70-9 | SDF | |
| 化学名 | S-[2,3-bis[(1-oxohexadecyl)oxy]propyl]-L-cysteinylglycyl-L-α-aspartyl-L-prolyl-L-lysyl-L-histidyl-L-prolyl-L-lysyl-L-seryl-L-phenylalanine | ||
| Canonical SMILES | N/A | ||
| 分子式 | C84H140N14O18S | 分子量 | 1666.2 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 600.2 μL | 3.0008 mL | 6.0017 mL |
| 5 mM | 120 μL | 600.2 μL | 1.2003 mL |
| 10 mM | 60 μL | 300.1 μL | 600.2 μL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
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