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Etoposide-d3 Sale

(Synonyms: EPE-d3, VP-16-123-d3) 目录号 : GC40195

An internal standard for the quantification of etoposide

Etoposide-d3 Chemical Structure

规格 价格 库存 购买数量
500μg
¥2,209.00
现货
1mg
¥4,196.00
现货

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Sample solution is provided at 25 µL, 10mM.

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Quality Control & SDS

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产品描述

Etoposide-d3 is intended for use as an internal standard for the quantification of etoposide by GC- or LC-MS. Etoposide is a plant alkaloid and an inhibitor of topoisomerase II (IC50 = 60.3 μM). It inhibits proliferation of a variety of adenocarcinoma cells (IC50s = 0.005-12,200 µM) and human umbilical vein endothelial (HUVEC) cells (IC50 = 0.249 µM). It reduces tumor growth in an Ma human embryonal carcinoma mouse xenograft model when administered at a dose of 25 mg/kg, an effect that is enhanced by concomitant administration of the immunosuppressant cyclosporin A . Etoposide also inhibits nuclear receptor coactivator 3 (IC50 = 2.48 µM). Formulations containing etoposide have been used in combination therapy in the treatment of cancer.

Chemical Properties

Cas No. SDF
别名 EPE-d3, VP-16-123-d3
分子式 C29H29D3O13 分子量 591.6
溶解度 Methanol: soluble 储存条件 Store at -20°C
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储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

溶解性数据

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1 mg 5 mg 10 mg
1 mM 1.6903 mL 8.4517 mL 16.9033 mL
5 mM 0.3381 mL 1.6903 mL 3.3807 mL
10 mM 0.169 mL 0.8452 mL 1.6903 mL
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Research Update

Simultaneous LC-MS/MS bioanalysis of etoposide and paclitaxel in mouse tissues and plasma after oral administration of self-microemulsifying drug-delivery systems

Biomed Chromatogr 2018 Jun;32(6):e4192.PMID:29349799DOI:10.1002/bmc.4192

In this study, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to simultaneously determine the anticancer drugs etoposide and paclitaxel in mouse plasma and tissues including liver, kidney, lung, heart, spleen and brain. The analytes were extracted from the matrices of interest by liquid-liquid extraction using methyl tert-butyl ether-dichloromethane (1:1, v/v). Chromatographic separation was achieved on an Ultimate XB-C18 column (100 × 2.1 mm, 3 μm) at 40°C and the total run time was 4 min under a gradient elution. Ionization was conducted using electrospray ionization in the positive mode. Stable isotope Etoposide-d3 and docetaxel were used as the internal standards. The lower limit of quantitation (LLOQ) of etoposide was 1 ng/g tissue for all tissues and 0.5 ng/mL for plasma. The LLOQ of paclitaxel was 0.4 ng/g tissue and 0.2 ng/mL for all tissues and plasma, respectively. The coefficients of correlation for all of the analytes in the tissues and plasma were >0.99. Both intra- and inter-day accuracy and precision were satisfactory. This method was successfully applied to measure plasma and tissue drug concentrations in mice treated with etoposide and paclitaxel-loaded self-microemulsifying drug-delivery systems.