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Dihydronovobiocin Sale

目录号 : GC47228

A coumarin antibiotic

Dihydronovobiocin Chemical Structure

Cas No.:29826-16-2

规格 价格 库存 购买数量
1 mg
¥2,552.00
现货
5 mg
¥10,210.00
现货

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产品描述

Dihydronovobiocin is a coumarin antibiotic and derivative of novobiocin .1 It is active against the bacteria S. aureus, S. haemolyticus, D. pneumoniae, S. typhosa, K. pneumoniae, and P. multocida (MICs = 0.6, 2, 0.6, 10, 10, and 3 µg/ml, respectively).2 Dihydronovobiocin inhibits DNA gyrase subunit B with an IC50 value of 64.5 nM.3

1.Berger, J., and Batcho, A.D.Coumarin - glycoside antibioticsJ. Chromatogr. Lib.15101-158(1978) 2.Hoeksema, H.Dihydronovobiocin and derivatives thereof3,175,9441-9(1965) 3.Gevi, M., and Domenici, E.A scintillation proximity assay amenable for screening and characterization of DNA gyrase B subunit inhibitorsAnal. Biochem.300(1)34-39(2002)

Chemical Properties

Cas No. 29826-16-2 SDF
Canonical SMILES O[C@H]1[C@H](OC2=CC=C(C(O)=C(NC(C3=CC(CCC(C)C)=C(O)C=C3)=O)C(O4)=O)C4=C2C)OC(C)(C)[C@H](OC)[C@H]1OC(N)=O
分子式 C31H38N2O11 分子量 614.6
溶解度 DMF: soluble,DMSO: soluble,Ethanol: soluble,Methanol: soluble 储存条件 Store at -20°C
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储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
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1 mg 5 mg 10 mg
1 mM 1.6271 mL 8.1354 mL 16.2707 mL
5 mM 0.3254 mL 1.6271 mL 3.2541 mL
10 mM 0.1627 mL 0.8135 mL 1.6271 mL
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Research Update

Demonstrating Ligandability of the LC3A and LC3B Adapter Interface

J Med Chem 2021 Apr 8;64(7):3720-3746.PMID:33769048DOI:10.1021/acs.jmedchem.0c01564.

Autophagy is the common name for a number of lysosome-based degradation pathways of cytosolic cargos. The key components of autophagy are members of Atg8 family proteins involved in almost all steps of the process, from autophagosome formation to their selective fusion with lysosomes. In this study, we show that the homologous members of the human Atg8 family proteins, LC3A and LC3B, are druggable by a small molecule inhibitor novobiocin. Structure-activity relationship (SAR) studies of the 4-hydroxy coumarin core scaffold were performed, supported by a crystal structure of the LC3A Dihydronovobiocin complex. The study reports the first nonpeptide inhibitors for these protein interaction targets and will lay the foundation for the development of more potent chemical probes for the Atg8 protein family which may also find applications for the development of autophagy-mediated degraders (AUTACs).

A scintillation proximity assay amenable for screening and characterization of DNA gyrase B subunit inhibitors

Anal Biochem 2002 Jan 1;300(1):34-9.PMID:11743689DOI:10.1006/abio.2001.5406.

DNA gyrase is the target of coumarin and cyclothialidine antibacterials, which bind to the B subunit of the enzyme (GyrB). Currently available GyrB inhibitors have not been clinically successful, but their high in vitro potency against DNA gyrase has raised interest in the development of novel noncoumarin antibacterials acting at the same site. We report the development of a simple scintillation proximity assay (SPA) for the study of binding interactions between coumarin or noncoumarin antibacterials and GyrB, which prevents the needs of separation steps and can be run in microtiter plate formats. The assay is based on the detection of the binding of a radioligand, [3H]Dihydronovobiocin, to a biotin-labeled 43-kDa fragment of GyrB (biotin-GyrB43), which is captured by streptavidin-coated SPA beads. The typical assay was conducted in 96-well microtiter plates, with final concentration of 10 nM for biotin-GyrB43, 20 nM for [3H]Dihydronovobiocin, and 33 microg of SPA beads/well. From saturation experiments, an equilibrium dissociation constant (K(d)) for Dihydronovobiocin of 8.10 nM was found. Displacement studies gave 50% inhibitory concentrations (IC(50)) of 42, 64, and 11 nM for novobiocin, Dihydronovobiocin, and the cyclothialidine analogue GR122222X, respectively, consistent with previous findings. The assay was found to be robust to dimethyl sulfoxide up to 5% (v/v) and can be used for high-throughput screens of large chemical collections in the search of novel DNA gyrase inhibitors.