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DC4 Crosslinker

目录号 : GC43384

A protein cross-linking reagent

DC4 Crosslinker Chemical Structure

Cas No.:1374647-94-5

规格 价格 库存 购买数量
5mg
¥857.00
现货
10mg
¥1,627.00
现货
25mg
¥3,854.00
现货

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Sample solution is provided at 25 µL, 10mM.

产品文档

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产品描述

DC4 crosslinker is a mass spectrometry-cleavable crosslinking reagent (18? in length) that contains two intrinsic positive charges, which allow cross-linked peptides to fragment into their component peptides by collision-induced dissociation or in-source decay. Initial fragmentation events result in cleavage on either side of the positive charges so that cross-linked peptides can be identified as pairs of ions separated by defined masses. Additionally, these two intact peptide fragments can be further fragmented to yield a series of b- and y-ions for peptide identification.

Chemical Properties

Cas No. 1374647-94-5 SDF
Canonical SMILES O=C(ON1C(CCC1=O)=O)CCC[N+]23CC[N+](CC3)(CCCC(ON4C(CCC4=O)=O)=O)CC2.[Br-].[Br-]
分子式 C22H32N4O8•2Br 分子量 640.3
溶解度 DMSO: 11 mg/ml,PBS (pH 7.2): 3 mg/ml 储存条件 Store at -20°C
General tips 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

溶解性数据

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1 mg 5 mg 10 mg
1 mM 1.5618 mL 7.8088 mL 15.6177 mL
5 mM 0.3124 mL 1.5618 mL 3.1235 mL
10 mM 0.1562 mL 0.7809 mL 1.5618 mL
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Research Update

Quaternary diamines as mass spectrometry cleavable crosslinkers for protein interactions

J Am Soc Mass Spectrom 2012 Feb;23(2):201-12.PMID:22131227DOI:PMC3573217

Mapping protein interactions and their dynamics is crucial to defining physiologic states, building effective models for understanding cell function, and to allow more effective targeting of new drugs. Crosslinking studies can estimate the proximity of proteins, determine sites of protein-protein interactions, and have the potential to provide a snapshot of dynamic interactions by covalently locking them in place for analysis. Several major challenges are associated with the use of crosslinkers in mass spectrometry, particularly in complex mixtures. We describe the synthesis and characterization of a MS-cleavable crosslinker containing cyclic amines, which address some of these challenges. The DC4 Crosslinker contains two intrinsic positive charges, which allow crosslinked peptides to fragment into their component peptides by collision-induced dissociation (CID) or in-source decay. Initial fragmentation events result in cleavage on either side of the positive charges so crosslinked peptides are identified as pairs of ions separated by defined masses. The structures of the component peptides can then be robustly determined by MS(3) because their fragmentation products rearrange to generate a mobile proton. The DC4 crosslinking reagent is stable to storage, highly reactive, highly soluble (1 M solutions), quite labile to CID, and MS(3) results in productive backbone fragmentation.