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DAF-2 目录号 GC43372

规格 价格 库存 购买数量
100μg
¥624.00
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250μg
¥1,482.00
现货
500μg
¥2,808.00
现货
1mg
¥4,992.00
现货

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Sample solution is provided at 25 µL, 10mM.

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Chemical Properties

Cas No. 205391-01-1 SDF Download SDF
别名 4,5-Diaminofluorescein
化学名 2-(3,6-dihydroxy-4,5-diamino-9H-xanthen-9-yl)-benzoic acid
Canonical SMILES OC1=CC2=C(C=C1)C3(C(C=C(N)C(N)=C4)=C4C(O3)=O)C5=CC=C(O)C=C5O2
分子式 C20H14N2O5 分子量 362.3
溶解度 Soluble in DMSO 储存条件 Store at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

产品描述

DAF-2 is a sensitive fluorescent indicator commonly used for the detection of nitric oxide (NO).[1],[2] It reacts with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein (DAF-2T). Fluorescence is monitored using excitation and emission wavelengths of 485 and 538 nM, respectively.2 At neutral pH the detection limit for NO is 2-5 nM.

Reference:

[1]. Kojima, H., Sakurai, K., Kikuchi, K., et al. Development of a fluorescent indicator for nitric oxide based on the fluorescein chromophore. Chemical & Pharmaceutical Bulletin 46, 373-375 (1998).
[2]. Nakatsubo, N., Kojima, H., Kikuchi, K., et al. Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: Diaminofluoresceins. FEBS Letters 427, 263-266 (1998).