Home>>Natural Products>>Dabsyl chloride (DABS-Cl)

Dabsyl chloride (DABS-Cl) Sale

(Synonyms: 磺酰氯; DABS-Cl) 目录号 : GC30077

Dabsyl chloride (DABS-Cl) 是一种胺衍生剂,能够产生稳定的产品,可以在 460 nm 分光光度法上轻松监测; Dabsyl chloride (DABS-Cl) 也用于标记氨基酸。

Dabsyl chloride (DABS-Cl) Chemical Structure

Cas No.:56512-49-3

规格 价格 库存 购买数量
50mg
¥270.00
现货

电话:400-920-5774 Email: sales@glpbio.cn

Customer Reviews

Based on customer reviews.

Sample solution is provided at 25 µL, 10mM.

产品文档

Quality Control & SDS

View current batch:

实验参考方法

Cell experiment:

Selected mouse brain samples from either cortical or striatal regions (100 mg wet weight) and neuroblastoma cells (SH-SY5Y) pellet derived from 25 cm2 flask are treated with 500 μL of 0.1 M HCl containing 0.2% TDGA, sonicated for 10 min (only for brain tissue), and then centrifuged at 14000 g for 30 min. The supernatant is freeze-dried. 50 μL of reaction buffer and 100 μL of 15 mM Dabsyl chloride are added to the tube and derivatized.

References:

[1]. Francioso A, et al. HPLC Determination of Bioactive Sulfur Compounds, Amino Acids and Biogenic Amines in Biological Specimens. Adv Exp Med Biol. 2017;975:535-549.

产品描述

Dabsyl chloride is an amine derivatizing agent, able to give rise to stable products that can be easily monitored spectrophotometrically at 460 nm; Dabsyl chloride also used for labeling amino acids.

Dabsyl chloride can give rise to mono-Dabsyl and bis-Dabsyl derivatives in the presence of multiple amino groups. Furthermore with respect to OPA derivatization, Dabsyl chloride can react with primary and also with secondary amines[1].

[1]. Francioso A, et al. HPLC Determination of Bioactive Sulfur Compounds, Amino Acids and Biogenic Amines in Biological Specimens. Adv Exp Med Biol. 2017;975:535-549.

Chemical Properties

Cas No. 56512-49-3 SDF
别名 磺酰氯; DABS-Cl
Canonical SMILES O=S(C1=CC=C(/N=N/C2=CC=C(N(C)C)C=C2)C=C1)(Cl)=O
分子式 C14H14ClN3O2S 分子量 323.8
溶解度 DMSO: ≥ 5.2 mg/mL (16.06 mM) 储存条件 Store at -20°C
General tips 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

溶解性数据

制备储备液
1 mg 5 mg 10 mg
1 mM 3.0883 mL 15.4416 mL 30.8833 mL
5 mM 0.6177 mL 3.0883 mL 6.1767 mL
10 mM 0.3088 mL 1.5442 mL 3.0883 mL
  • 摩尔浓度计算器

  • 稀释计算器

  • 分子量计算器

质量
=
浓度
x
体积
x
分子量
 
 
 
*在配置溶液时,请务必参考产品标签上、MSDS / COA(可在Glpbio的产品页面获得)批次特异的分子量使用本工具。

计算

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % saline
计算重置

Research Update

Reversed-phase high-performance liquid chromatographic analysis of hydroxyproline and proline from collagen by derivatization with Dabsyl chloride

J Chromatogr 1993 Nov 24;621(2):133-8.8294534 10.1016/0378-4347(93)80088-l

A high-performance liquid chromatographic method for the analysis of hydroxyproline and proline has been developed. The method is based on the derivatization of the secondary amino group with dabsyl-chloride after blocking of the primary amino group with o-phthalaldehyde. Dabsyl-hydroxyproline and dabsyl-proline were separated from other amino acids by high-performance liquid chromatography in the gradient elution mode, and eluted at 10.27 and 16.02 min, respectively. The correlations between the peak areas of dabsyl-hydroxyproline and dabsyl-proline were linear in the range from 20-200 pmol, with equations y = 1.10x - 0.80 (r = 0.999) and y = 1.12x - 0.52 (r = 0.999), respectively. The method was applied to the analysis of rat tail collagen, and the contents of hydroxyproline and proline were 1.55 +/- 0.04 and 2.03 +/- 0.04 nmol/micrograms, respectively.

Determination of neomycin in the form of neomycin derivative with Dabsyl chloride by thin layer chromatography and densitometry

Acta Pol Pharm 2015 Jan-Feb;72(1):31-7.25850198

A thin layer chromatographic-densitometric method has been developed for identification and quantitative determination of neomycin derivative with Dabsyl chloride. The analysis of antibiotic was achieved on the silica gel TLC plates with fluorescent indicator with n-butanol--2-butanone--25% ammonia--water (10 : 6 : 2 : 2, v/v/v/v) as the mobile phase. The densitometric measurements were made at 460 nm. Under these conditions good separation of chosen aminoglycoside antibiotic from reagent used to make a complex was obtained. The method is characterized by high sensitivity, LOD from 0.1953 μg per band and LOQ from 0.5918 μg per band, wide linearity range from 0.5918 to 2.1960 μg per band for neomycin. The precision of the method was good; RSD varied from 1.17 to 2.05%. Satisfactory results of validation of the method were also confirmed by determination of selected antibiotic in pharmaceutical commercial preparation. The results obtained by TLC-densitometric method were compared with those obtained by spectrophotometric method.

Visible labeling of proteins for polyacrylamide gel electrophoresis with Dabsyl chloride

Anal Biochem 1984 Aug 15;141(1):121-6.6437269 10.1016/0003-2697(84)90434-2

Several proteins, which are used as molecular weight markers in polyacrylamide gel electrophoresis, were reacted with Dabsyl chloride. This labeled them deep orange and the chromophore attachment was stable throughout the electrophoretic procedure and fixation. Small amounts (10-50 micrograms) of the labeled proteins could be loaded onto gels and seen with the unaided eye so that the separation during electrophoresis could be followed. Dabsylation did not affect the mobility of the proteins. The location of the orange band gave a good indication of the position of the protein in the gel so that molecular weight estimations could be made during and immediately following electrophoresis.

Determination of taurine and hypotaurine in animal tissues by reversed-phase high-performance liquid chromatography after derivatization with Dabsyl chloride

Adv Exp Med Biol 2003;526:221-8.12908604 10.1007/978-1-4615-0077-3_28

Spectrophotometric determination of bacitracin in bulk drug as dabsyl derivative in a range of visible light

Acta Pol Pharm 2011 Nov-Dec;68(6):853-8.22125949

A fast spectrophotometric method has been developed for bacitracin identification and determination after condensation reaction with Dabsyl chloride. In addition, determination of dye stability of sulfonamide derivative and identification of the molar ratio of reagents was done at various time-points. The developed method has a good linearity with very broad spectrum, correlation coefficient of r = 0.9972, good precision (RSD = 1.54 +/- 0.11%), and recovery at three different levels of concentration was found between 98.33% and 103.47%. Usefulness of the method was demonstrated by positive results obtained during determination of bacitracin concentration in bulk drug.