Cardiogenol C hydrochloride
(Synonyms: 2-[[2-[(4-甲氧基苯基)氨基]-4-嘧啶基]氨基]乙醇盐酸盐) 目录号 : GC17840
Cardiogenol C hydrochloride 是一种有效的细胞渗透性嘧啶诱导剂，可促进 ESC 分化为心肌细胞 (EC50=100 nM)。
Cas No.:1049741-55-0
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cardiogenol C hydrochloride is a cell-permeable pyrimidine compound which potently induces the differentiation of ESCs into cardiomyocytes (EC50= 100 nM).IC50 value: 100 nM (EC50)Target:in vitro: Cardiogenol C hydrochloride is a cardiomyogenesis inducer in embryonic stem cells. Cardiogenol C induces the differentiation of myosin heavy chain-positive cardiomyocytes from embryonic stem cells with an EC50 value of 0.1 μM; about 90% of embryonic stem cells treated with 0.25 μM of Cardiogenol C express the cardiac muscle cell specific transcription factors GATA-4, MEF2, and Nkx2.5 and display the characteristic beating behavior of differentiated cardiomyocytes. Cardiogenol C (a diaminopyrimidine) induces cardiac differentiation in P19 and in P19Cl6 cells. [1] Cardiogenol C could activate Wnt/β-catenin signaling to induce cardiogenesis. Cardiogenol C-treatment significantly decreased HBPCs proliferation. Cardiogenol C was able to induce HBPCs to transdifferentiate into cardiomyocyte-like cells.[2]
References:
[1]. Jasmin, et al. Chemical induction of cardiac differentiation in p19 embryonal carcinoma stem cells. Stem Cells Dev. 2010 Mar;19(3):403-412.
[2]. Yau WW, et al. Cardiogenol C can induce Mouse Hair Bulge Progenitor Cells to Transdifferentiate into Cardiomyocyte-like Cells. Proteome Sci. 2011 Jan 19;9(1):3.
| Cas No. | 1049741-55-0 | SDF | |
| 别名 | 2-[[2-[(4-甲氧基苯基)氨基]-4-嘧啶基]氨基]乙醇盐酸盐 | ||
| 化学名 | 2-((2-((4-methoxyphenyl)amino)pyrimidin-4-yl)amino)ethanol hydrochloride | ||
| Canonical SMILES | OCCNC1=NC(NC(C=C2)=CC=C2OC)=NC=C1.Cl | ||
| 分子式 | C13H16N4O2.HCl | 分子量 | 296.75 |
| 溶解度 | ≥ 14.85mg/mL in Water with ultrasonic | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.3698 mL | 16.8492 mL | 33.6984 mL |
| 5 mM | 0.674 mL | 3.3698 mL | 6.7397 mL |
| 10 mM | 0.337 mL | 1.6849 mL | 3.3698 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。