Calmagite
(Synonyms: 钙镁试剂) 目录号 : GC33507
Calmagite是一个络合指示剂，可用于检测各种样品中的钙和镁。
Cas No.:3147-14-6
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Calmagite is a complexometric indicator which can be used to detect calcium and magnesium in various samples.
[1]. Rasouli Z, et al. Simultaneously detection of calcium and magnesium in various samples by calmagite and chemometrics data processing. Spectrochim Acta A Mol Biomol Spectrosc. 2016 Dec 5;169:72-81.
| Cas No. | 3147-14-6 | SDF | |
| 别名 | 钙镁试剂 | ||
| Canonical SMILES | O=S(C1=C2C=CC=CC2=C(/N=N/C3=CC(C)=CC=C3O)C(O)=C1)(O)=O | ||
| 分子式 | C17H14N2O5S | 分子量 | 358.37 |
| 溶解度 | DMSO : 6 mg/mL (16.74 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.7904 mL | 13.9521 mL | 27.9041 mL |
| 5 mM | 0.5581 mL | 2.7904 mL | 5.5808 mL |
| 10 mM | 0.279 mL | 1.3952 mL | 2.7904 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Calmagite as a spectrophotometric reagent for aluminium
Talanta 1968 Mar;15(3):321-5.PMID:18960299DOI:10.1016/0039-9140(68)80062-1.
Calmagite is proposed as a sensitive spectrophotometric reagent for aluminium, (570mmu) = 42000. After aqueous phase reaction at pH 8.6, the metal-reagent complex is extracted into chloroform by formation of an ion-association complex with a quaternary ammonium salt. The method is free from interference by common anions, and cationic interferences may be eliminated by the use of cyanide and EDTA as masking agents.
The oxidative degradation of Calmagite using added and in situ generated hydrogen peroxide catalysed by manganese(II) ions: Efficacy evaluation, kinetics study and degradation pathways
Chemosphere 2022 Jan;286(Pt 2):131792.PMID:34388875DOI:10.1016/j.chemosphere.2021.131792.
Manganese (II) ions (Mn(II)) catalyse the oxidative degradation of Calmagite (CAL, 2-hydroxy-1-(2-hydroxy-5methylphenylazo)-4-naphthalenesulfonic acid) at room temperature using added and in situ generated hydrogen peroxide (H2O2), using 1,2-dihydroxybenzene-3,5-disulfonate, disodium salt and monohydrate (Tiron) as the co-catalyst for the in situ generation of H2O2. The percentage of CAL degradation with the in situ generated H2O2 was 91.1 % after 30 min which is lower than that in the added H2O2/Mn(II) system (96.0 %). A one-eighth-lives method was applied to investigate the kinetic parameters in the added H2O2 system, with and without Mn(II), involving phosphate, carbonate, and two biological buffers at different pHs. Percarbonate (HCO4-) was found to be the main reactive species for CAL degradation in the added H2O2 system buffered by carbonate in the absence of Mn(II). Manganese (IV) = O (Mn(IV) = O) and manganese(V) = O (Mn(V) = O) are the main reactive species in the added H2O2/Mn(II) system buffered by carbonate and non-carbonate buffers respectively. pH 8.5 was the optimum pH for CAL degradation when buffered by carbonate, while pH 10.0 is the best pH for the systems not using carbonate buffer. Using a high performance liquid chromatography/electrospray ionisation mass spectrometer (HPLC/ESI-MS), the degradation intermediates of CAL were identified as 1-amino-2-naphthol-4-sulfonate ion, 1-amino-2-naphthol-4-sulfinic ion, 1-amino-2-naphthol, and 1-nitroso-2-naphthol.
Simultaneously detection of calcium and magnesium in various samples by Calmagite and chemometrics data processing
Spectrochim Acta A Mol Biomol Spectrosc 2016 Dec 5;169:72-81.PMID:27341399DOI:10.1016/j.saa.2016.06.027.
The current study describes results of the application of radial basis function-partial least squares (RBF-PLS), partial robust M-regression (PRM), singular value decomposition (SVD), evolving factor analysis (EFA), multivariate curve resolution with alternating least squares (MCR-ALS) and rank annihilation factor analysis (RAFA) methods for the purposes of simultaneous determination of trace amounts calcium (Ca(2+)) and magnesium (Mg(2+)) and exploratory analysis based on their colored complexes formation with 1-(1-hydroxy-4-methyl-2-phenylazo)-2-naphthol-4-sulfonic acid (Calmagite) as chromomeric reagent. The complex formation Ca(2+) and Mg(2+) with Calmagite was investigated under pH10.20. The performance of RBF-PLS model in detection of minerals was compared with PRM as a linear model. The pure concentration and spectral profiles were obtained using MCR-ALS. EFA and SVD were used to distinguish the number species. The stability constants of the complexes were derived using RAFA. Finally, RBF-PLS was utilized for simultaneous determination of minerals in pharmaceutical formulation and various vegetable samples.
Automated Calmagite compleximetric measurement of magnesium in serum, with sequential addition of EDTA to eliminate endogenous interference
Clin Chem 1984 Nov;30(11):1801-4.PMID:6435911doi
We describe an automated method for magnesium assay, for use with the Cobas-Bio centrifugal analyzer. Magnesium is reacted with Calmagite, a dye, and the absorbance of the magnesium-dye complex at 520 nm is measured. EDTA is then added to break up the complex and the absorbance at 520 nm is re-measured to correct for sample color and turbidity. Including ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) and triethanolamine in the dye reagent eliminates interference by calcium and iron. Within-run CVs were less than 2.0% for concentrations of magnesium ranging from 18 to 40 mg/L, and less than 4% for a magnesium concentration of 9.4 mg/L. Day-to-day precision data, determined over five months were: mean = 21.0 mg/L, CV = 2.7%; mean = 43.0 mg/L, CV = 3.2% (n = 550 for both). Comparison of the Cobas-Bio method (y) with an atomic absorption spectrometric method (x) gave the following results: y = 0.968x + 0.448, r = 0.986, Sy/x = 0.34 mg/L, mean x = 19.8 mg/L, mean y = 19.6 mg/L, n = 44. Hemoglobin, bilirubin, and turbidity do not interfere. The standard curve is linear up to a magnesium concentration of 97 mg/L.
Assessment of the functionality of a pilot-scale reactor and its potential for electrochemical degradation of Calmagite, a sulfonated azo-dye
Chemosphere 2008 Oct;73(5):837-43.PMID:18676003DOI:10.1016/j.chemosphere.2008.06.050.
Electrochemical degradation (ECD) is a promising technology for in situ remediation of diversely contaminated environmental matrices by application of a low level electric potential gradient. This investigation, prompted by successful bench-scale ECD of trichloroethylene, involved development, parametric characterization and evaluation of a pilot-scale electrochemical reactor for degradation of Calmagite, a sulfonated azo-dye used as a model contaminant. The reactor has two chambers filled with granulated graphite for electrodes. The system has electrical potential, current, conductivity, pH, temperature, water-level and flow sensors for automated monitoring. The reactor supports outdoor and fail-safe venting, argon purging, temperature regulation and auto-shutdown for safety. Treatment involves recirculating the contaminated solution through the electrode beds at small flow velocities mimicking low fluid-flux in groundwater and submarine sediments. The first phase of the investigation involved testing of the reactor components, its parametric probes and the automated data acquisition system for performance as designed. The results showed hydraulic stability, consistent pH behavior, marginal temperature rise (<5 degrees C) and overall safe and predictable performance under diverse conditions. Near complete removal of Calmagite was seen at 3-10V of applied voltage in 8-10h. The effects of voltage and strength of electrolyte on degradation kinetics have been presented. Further, it was observed from the absorption spectra that as Calmagite degrades over time, new peaks appear. These peaks were associated with degradation products identified using electrospray ionization mass spectrometry. A reaction mechanism for ECD of Calmagite has also been proposed.