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C11 BODIPY 581/591 目录号 GC40165

C11 BODIPY 581/591 is a lipid-soluble ratiometric fluorescent indicator of lipid oxidation

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1mg
¥1,950.00
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Sample solution is provided at 25 µL, 10mM.

质量管理

Quality Control & SDS

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实验参考方法

Cell experiment [1]:

Cell lines

microalgal cells (unicellular green alga Chlamydomonas reinhardtii); mammalian cells

Preparation method

The stock solution of C11-BODIPY581/591 was prepared by dissolving 1 mg of the product in DMSO to a concentration of 1mM. Stock solution can be stored in freezer (-5 to -30°C) and protect from light.

Reaction Conditions

2.5 µM C11-BODIPY581/591 for 30 min. The excitation and emission band of oxidized type is pass of 460–495 and 510–550, respectively. But the excitation and emission band of reduced type is pass of 565–581 and 585–591, respectively.

Applications

C11-BODIPY591/581 staining was combined with flow cytometry measurements to detect reactive oxygen species (ROS) and oxidative stress damage in living cells and membrane systems. Oxidation of the polyunsaturated butadienyl portion of the dye results in a shift of the fluorescence emission peak from ~590 nm to ~510 nm.

References:

[1]. Giulia Cheloni, Vera I. Slaveykova. Optimization of the C11-BODIPY581/591 Dye for the Determination of Lipid Oxidation in Chlamydomonas reinhardtii by Flow Cytometry. Cytometry A,2013 Oct;83(10):952-61..

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Chemical Properties

Cas No. 217075-36-0 SDF Download SDF
别名 N/A
化学名 (T-4)-difluoro[5-[[5-[(1E,3E)-4-phenyl-1,3-butadien-1-yl]-2H-pyrrol-2-ylidene-κN]methyl]-1H-pyrrole-2-undecanoato(2-)-κN1]-borate(1-), monohydrogen
Canonical SMILES [F-][B+3]1([N]2=C(/C=C/C=C/C3=CC=CC=C3)C=CC2=CC4=CC=C(CCCCCCCCCCC([O-])=O)[N-]14)[F-].[H+]
分子式 C30H34BF2N2O2 • H 分子量 504.4
溶解度 Soluble in DMSO 储存条件 Store at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

产品描述

C11 BODIPY 581/591 is a lipid-soluble ratiometric fluorescent indicator of lipid oxidation.[1] Upon oxidation, its excitation maximum shifts from 581 to 500 nm and the emission maximum shifts from 591 to 510 nm. C11 BODIPY 581/591 has been used in biochemical assays and live cell applications.[1],[2]

Reference:

[1]. Pap, E.H.W., Drummen, G.P.C., Winter, V.J., et al. Ratio-fluorescence microscopy of lipid oxidation in living cells using C11-BODIPY581/591 FEBS Lett. 453(3), 278-282 (1999).
[2]. Naguib, Y.M.A. A fluorometric method for measurement of peroxyl radical scavenging activities of lipophilic antioxidants Anal. Biochem. 265(2), 290-298 (1998).