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Biotin-tyramide 目录号 GC13376

Reagent used for tyramide signal amplification (TSA)

规格 价格 库存 购买数量
100mg
¥714.00
现货
1g
¥5,765.00
现货

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Sample solution is provided at 25 µL, 10mM.

质量管理

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Chemical Properties

Cas No. 41994-02-9 SDF
别名 Biotin-Phenol,Biotin Phenol
化学名 N-(4-hydroxyphenethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide
Canonical SMILES O=C1N[C@@]([H])([C@]([H])(CS2)N1)[C@@H]2CCCCC(NCCC3=CC=C(O)C=C3)=O
分子式 C18H25N3O3S 分子量 363.47
溶解度 ≥ 72.6mg/mL in DMSO, ≥ 8.18 mg/mL in EtOH with ultrasonic 储存条件 Store at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
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产品描述

Biotin-tyramide is used for tyramide signal amplification (TSA) which is a powerful, patented technology that significantly enhances both chromogenic and fluorescent signals. TSA is easily integrated into standard nonradioactive in situ hybridization (ISH) or IHC protocols.

TSA is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to catalyze the deposition of a fluorophore-labeled tyramide amplification reagent onto tissue sections or cell preparation surfaces that have been previously blocked with proteins.

Biotin-tyramide use horseradish peroxidase (HRP) to catalyze covalent deposition of biotin labels directly adjacent to the immobilized enzyme. The labeling reaction is quick (less than 10 minutes) and deposited labels can be detected with streptavidin conjugates for imaging in brightfield or fluorescence microscopy.