BHQ
(Synonyms: 2,5-二特丁基对苯二酚) 目录号 : GC14664
间接食品添加剂 BHQ (DTBHQ) 调节 5-脂氧合酶的活性以及 COX-2 的活性（IC50=1.8 和 14.1 ⋼；M 分别代表 5-LO 和 COX-2）。
Cas No.:88-58-4
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
BHQ is a selective inhibitor of endoplasmic reticulum Ca2+-ATPase.
Endoplasmic reticulum Ca2+-ATPase (SR Ca2+-ATPase) is a Ca2+-ATPase and transfers Ca2+ from the cytosol of the cell to the lumen of the sarcoplasmic reticulum (SR) during muscle relaxation.
BHQ is a selective SR Ca2+-ATPase inhibitor. In rat basophilic leukaemia cells, BHQ (10 μM) blocked inward rectifier potassium current and might cause depolarization of the cell and affect Ca2+ influx [1]. In aortic rings at rest or depolarised with 80 mM K+ in the presence of 1 mM nifedipine, BHQ induced a slow tonic contraction. At 20 mM K+, BHQ increased Ca2+-induced contraction. However, BHQ inhibited Ca2+-induced contraction at 40, 80 and 128 mM K+ [2]. In smooth muscle cells from the rat tail artery, BHQ reduced L-type Ca2+ current (ICa(L)) with IC50 value of 66.7 μM in a concentration- and voltage-dependent way. BHQ increased superoxide anion formation, which was markedly inhibited by superoxide dismutase (SOD). These results suggested that BHQ inhibited ICa(L) by the generation of superoxide anion [3]. In Madin Darby canine kidney (MDCK) cells, BHQ increased [Ca2+]i with EC50 value of 40 μM in a dose-dependent way, which was contributed by depleting the endoplasmic reticulum Ca2+ store followed by capacitative Ca2+ entry [4].
References:
[1]. Hasséssian H, Vaca L, Kunze DL. Blockade of the inward rectifier potassium current by the Ca(2+)-ATPase inhibitor 2',5'-di(tert-butyl)-1,4-benzohydroquinone (BHQ). Br J Pharmacol, 1994, 112(4): 1118-1122.
[2]. Fusi F, Gorelli B, Valoti M, et al. Effects of 2,5-di-t-butyl-1,4-benzohydroquinone (BHQ) on rat aorta smooth muscle. Eur J Pharmacol, 1998, 346(2-3): 237-243.
[3]. Fusi F, Saponara S, Gagov H, et al. 2,5-Di-t-butyl-1,4-benzohydroquinone (BHQ) inhibits vascular L-type Ca(2+) channel via superoxide anion generation. Br J Pharmacol, 2001, 133(7): 988-996.
[4]. Jan CR, Ho CM, Wu SN, et al. Mechanism of rise and decay of 2,5-di-tert-butylhydroquinone-induced Ca2+ signals in Madin Darby canine kidney cells. Eur J Pharmacol, 1999, 365(1): 111-117.
| Cas No. | 88-58-4 | SDF | |
| 别名 | 2,5-二特丁基对苯二酚 | ||
| 化学名 | 2,5-di-tert-butylbenzene-1,4-diol | ||
| Canonical SMILES | OC(C(C(C)(C)C)=C1)=CC(C(C)(C)C)=C1O | ||
| 分子式 | C14H22O2 | 分子量 | 222.33 |
| 溶解度 | ≥ 8mg/mL in DMSO | 储存条件 | Store at RT |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.4978 mL | 22.4891 mL | 44.9782 mL |
| 5 mM | 0.8996 mL | 4.4978 mL | 8.9956 mL |
| 10 mM | 0.4498 mL | 2.2489 mL | 4.4978 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。