BCA Protein Assay Kit (BCA蛋白浓度测定试剂盒)

The BCA Protein Assay Kit is a ready-to-use total protein analysis reagent that can quickly determine total protein concentration by measuring the absorbance at 562 nm and comparing it with the absorbance-concentration curve of a protein standard.

This kit is suitable for measuring the total protein content of purified proteins, animal and plant tissue samples, and various cell samples. The detection concentration range is 20-2000 μg/mL. As the protein concentration increases, the absorbance has a linear relationship with the protein concentration.

This kit is developed based on the BCA (Bicin-choninic Acid) method, which enables fast, stable and sensitive concentration determination of protein. The principle is that under alkaline conditions, the protein reduces Cu²⁺ to Cu⁺, and Cu⁺ forms a purple complex with the BCA reagent. This water-soluble complex shows strong absorbance at 562 nm.

If you need to accurately quantify the protein to be tested, it is recommended to choose a protein standard with a quality similar to that of the protein to be tested. For example, when measuring immunoglobulin samples, bovine gamma globulin (BGG) standards can be used.

In addition, this kit provides a series of concentration gradient BSA (Bovine Serum Albumin, bovine serum albumin) standards with a concentration range of 150-2000 μg/mL, eliminating the need for a series of gradient dilution processes and making the detection process faster.

BCA Protein Assay Kit是一种即用型的总蛋白分析试剂, 可通过测量562 nm处的吸光度并与蛋白标准物的吸光度-浓度曲线进行比较, 快速测定总蛋白浓度。

本试剂盒适用于对纯化蛋白、动植物组织样本以及各类细胞样本进行总蛋白含量测定，检测浓度范围20-2000μg/mL。随着蛋白质浓度的增加, 吸光度与蛋白浓度呈线性关系。

本试剂盒是基于BCA (Bicin-choninic Acid) 法研制而成, 实现了对蛋白质进行快速、稳定、灵敏的浓度测定。 其原理是在碱性条件下，蛋白将Cu²⁺还原成Cu⁺， Cu⁺与BCA试剂形成紫色的络合物，这种水溶性复合物在562 nm处表现出很强的吸光度。

如果需要精确定量待测蛋白质, 建议选择质量与待测蛋白质相似的蛋白质标准品。例如, 在测定免疫球蛋白样品时, 可以使用牛γ球蛋白 (BGG) 标准品。

此外，本试剂盒提供一系列浓度梯度的BSA（Bovine Serum Albumin，牛血清白蛋白）标准品，浓度范围150-2000μg/mL，无需进行一系列梯度稀释过程，检测过程更加快捷。