Azoxymethane
(Synonyms: 偶氮甲烷; AOM) 目录号 : GC19445
偶氮甲烷作为一种结肠致癌物,可导致DNA加合物的形成。偶氮甲烷(AOM)导致O(6)-甲基鸟嘌呤加合物的形成,从而导致G->A跃迁。.
Cas No.:25843-45-2
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
| Cell experiment [1]: | |
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Cell lines |
hepatic microsomes |
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Preparation Method |
The assay for AOM-induced in vitro DNA adduct formation was based on a published method. Briefly, microsomes (0.5-2.0 mg/mL) were incubated with calf thymus DNA (1 mg/mL) and AOM (200 µM) in a total volume of 1.0 mL. The assay buffer consisted of 0.1 M Tris-HCl (pH 7.4), 1 mM EDTA, 20 mM MgCl2, 0.3 M KCl, and 1.5 mM NADPH. Incubations were carried out at 37 °C for 60 min in a shaking water bath. An additional 30 nmol of NADPH was added after the first 30 min. The reaction was stopped by the addition of 0.5 mL of ice-cold 7.5 M ammonium acetate. DNA was then extracted as described above for tissue homogenates. Control incubations were performed without NADPH. |
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Reaction Conditions |
200 µM; 60 min |
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Applications |
On an equal protein basis, hepatic microsomes were much more active than SI and colon microsomes in NADPH-dependent AOM bioactivation and N7-mG adduct formation. Microsomes of the liver (but not those of the SI or colon) of LCN mice showed significantly lower activity in N7-mG formation compared to that of WT mice. |
| Animal experiment [2]: | |
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Animal models |
8 to 10-week-old male C57BL/6J mice |
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Preparation Method |
On day 0, mark and weight each mice. Prepare 1 mg/mL (1 µg/µL) AOM working solution in PBS, and inject each mouse intraperitoneally with 10 mg/kg (body weight) of AOM (For example, a 20 g mouse shall be injected with 200 µL AOM solution). Then each mice was providedwith 2.5% DSS-containing drinking water for 7 days, followed with normal water for 10 days. This cycle of DSS -containing water and normal water shall be repeated three times, and then the normal water treatment stays last to the experimental endpoint. |
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Dosage form |
10 mg/kg, i.p. |
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Applications |
The colitis-associated CRC (colorectal cancer) model in mice can be made by using AOM and DSS. AOM- and DSS-induced murine colorectal cancer model has a body weight dynamics. DSS administration could cause significant weight loss. |
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References: [1] Megaraj V, et al. Role of hepatic and intestinal p450 enzymes in the metabolic activation of the colon carcinogen azoxymethane in mice. Chem Res Toxicol. 2014 Apr 21;27(4):656-62. |
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Azoxymethane, as a colon carcinogen, can lead to the formation of DNA adducts[1]. Azoxymethane (AOM) causes O(6)-methylguanine adduct formation which leads to G-->A transitions[2].
In vivo, 7-week-old conventional male C57BL/6 mice were injected intraperitoneally with 6 doses of 10 mg/kg AOM, feces from obese individuals promote colorectal cancer formation in AOM-treated CRC mouse model[3]. SWR/J, A/J mice was treated with 10 mg/kg AOM i.p. once a week for 8 weeks developed tumors, with a distribution that was limited to the distal colon[4]. In wild-type (WT) mice, AOM treatment (10 mg/kg) induced a 2.5-fold increase in intestinal crypt stem cell survival. WT mice receiving AOM exhibited increased intestinal apoptosis and a simultaneous reduction in crypt mitotic figures within 8 h of injection[5].
[1] Chen W, et al. Effect of tea on the formation of DNA adducts by azoxymethane. Xenobiotica. 1998 Feb;28(2):213-7.
[2] Wali RK, et al. Inhibition of O(6)-methylguanine-DNA methyltransferase increases azoxymethane-induced colonic tumors in rats. Carcinogenesis. 1999 Dec;20(12):2355-60.
[3] Kang X, et al. Altered gut microbiota of obesity subjects promotes colorectal carcinogenesis in mice. EBioMedicine. 2023 Jul;93:104670.
[4] Papanikolaou A, et al. Azoxymethane-induced colon tumors and aberrant crypt foci in mice of different genetic susceptibility. Cancer Lett. 1998 Aug 14;130(1-2):29-34.
[5] Riehl TE, et al. Azoxymethane protects intestinal stem cells and reduces crypt epithelial mitosis through a COX-1-dependent mechanism. Am J Physiol Gastrointest Liver Physiol. 2006 Dec;291(6):G1062-70.
References:
偶氮甲烷作为一种结肠致癌物,可导致DNA加合物的形成[1]。偶氮甲烷(AOM)导致O(6)-甲基鸟嘌呤加合物的形成,从而导致G->A跃迁[2]。
在体内,7周龄的常规雄性C57BL/6小鼠腹膜内注射6剂量的10 mg/kg AOM,来自肥胖个体的粪便促进AOM治疗的CRC小鼠模型中结直肠癌癌症的形成[3]。SWR/J,A/J小鼠用10 mg/kg AOM腹腔注射治疗,每周一次,持续8周,出现肿瘤,其分布仅限于远端结肠[4]。在野生型(WT)小鼠中,AOM处理(10 mg/kg)诱导肠隐窝干细胞存活率增加2.5倍。接受AOM的WT小鼠在注射后8小时内表现出肠细胞凋亡增加和隐窝有丝分裂图同时减少[5]。
| Cas No. | 25843-45-2 | SDF | |
| 别名 | 偶氮甲烷; AOM | ||
| Canonical SMILES | C/[N+]([O-])=N/C | ||
| 分子式 | C2H6N2O | 分子量 | 74.08 |
| 溶解度 | Soluble in water, ethanol, and ether | 储存条件 | Store at -20°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 13.4989 mL | 67.4946 mL | 134.9892 mL |
| 5 mM | 2.6998 mL | 13.4989 mL | 26.9978 mL |
| 10 mM | 1.3499 mL | 6.7495 mL | 13.4989 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。