Adenine HCl
(Synonyms: 腺嘌呤盐酸盐; 6-Aminopurine hydrochloride; Vitamin B4 hydrochloride) 目录号 : GC11825
Adenine HCl (6-Aminopurine hydrochloride) 是一种嘌呤，是 DNA 核酸中的四种核碱基之一。盐酸腺嘌呤充当 DNA 和 RNA 的化学成分。腺嘌呤 HCl 在涉及细胞呼吸、ATP 和辅因子（NAD 和 FAD）的形式以及蛋白质合成的生物化学中也发挥着重要作用。
Cas No.:2922-28-3
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
IC50: Not available.
Adenine HCl, a purine derivative and a nucleobase, plays crucial roles in substantial biochemistry processes in vivo, including cellular respiration, formation of the energy-rich adenosine triphosphate (ATP), the cofactors nicotinamide adenine dinucleotide (NAD) and flavin adenine dinucleotide (FAD) as well as protein synthesis. In addition, Adenine HCl also serves as a chemical component of DNA and RNA. Adenosine triphosphate is used in cellular metabolism as one of the basic methods of transferring chemical energy between chemical reactions. [1]
In vitro: The cyto-protective effect of Adenosine was measured using an in vitro model of acute tubular necrosis in rat kidney tubular cells. The finding suggested that Adenosine at the concentration of 100 M could significantly decrease cellular injury. The EC50 value of Adenosine was detected to be 14 M. [2]
In vivo: A study was performed to investigate the effects of dietary adenine on fatty liver induced by orotic acid (OA) in rats. 1% OA-supplemented diets with/ without 0.25% adenine was administered to rats for 10 days. Enzyme assay kits were then applied to measure serum lipid profiles of tested rats, such as liver lipid concentrations in different treatment groups. Moreover, the activities of fatty acid synthase (FAS) and fatty acid β-oxidation were also detected. The findings suggested that addition of adenine to the diet offset the effect of OA and reversed promotion of liver TG content to basal level. Administration of Adenine also inhibited FAS activities in rat liver. In conclusion, the ameliorating of fatty liver in adenine-treated rats was associated with the reduction of FAS activities accompanied with the increase of mitochondrial fatty acid β-oxidation and the promotion of serum lipid secretion from the hepatic tissue into the bloodstream. [1]
Clinical trials: So far, no clinical trial has been conducted.
References:
[1]Buang Y. Dietary adenine alleviates fatty liver induced by orotic acid. Indo. J. Chem. 2010; 10 (3): 363 - 369.
[2] Módis K, Ger D, Nagy N, Szoleczky P, Tóth ZD and Szabó C. Cytoprotective effects of adenosine and inosine in an in vitro model of acute tubular necrosis. Br J Pharmacol. 2009 Nov; 158(6): 1565–8.
| Cas No. | 2922-28-3 | SDF | |
| 别名 | 腺嘌呤盐酸盐; 6-Aminopurine hydrochloride; Vitamin B4 hydrochloride | ||
| 化学名 | 7H-purin-6-amine;hydrochloride | ||
| Canonical SMILES | C1=NC2=C(N1)C(=NC=N2)N.Cl | ||
| 分子式 | C5H5N5.HCl | 分子量 | 171.59 |
| 溶解度 | ≥ 11.5mg/mL in DMSO with gentle warming, ≥ 6.91mg/mL in Water with gentle warming | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 5.8278 mL | 29.1392 mL | 58.2785 mL |
| 5 mM | 1.1656 mL | 5.8278 mL | 11.6557 mL |
| 10 mM | 0.5828 mL | 2.9139 mL | 5.8278 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。