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Acetylcysteine 目录号 GC11786

Antioxidant;mucolytic agent

规格 价格 库存 购买数量
10mM (in 1mL DMSO)
¥420.00
现货
5g
¥389.00
现货
10g
¥431.00
现货

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Sample solution is provided at 25 µL, 10mM.

质量管理

Quality Control & SDS

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实验参考方法

Cell experiment [1]:

Cell lines

PC12 cells

Preparation method

The solubility of this compound in DMSO is > 10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

1–1000 μM; 3h

Applications

In PC12 cells, Acetylcysteine (NAC) produced concentration-dependent decreases in DOPAL levels. Co-incubation of NAC (1-10 μM) with selegiline (1 μM) attenuated or prevented the Cys-DA response to selegiline, without interfering with the selegiline-induced decrease in DOPAL production or inhibiting tyrosine hydroxylation.

Animal experiment [2]:

Animal models

the R6/1 transgenic mouse model of Huntington’s disease (HD)

Dosage form

500 mg/kg i.p., 4 weeks

Application

In the R6/1 transgenic mouse model of Huntington’s disease (HD), Acetylcysteine (NAC)-HD mice showed forced-swim test (FST) immobility times intermediate to saline-HD and saline-WT mice. NAC exhibited antidepressant-like effect. HD mice co-administered with CPG (inhibitor of GLT-1) and NAC showed higher immobility times than NAC-HD mice, suggesting that the antidepressant-like effect of NAC is dependent on glutamate transport.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Goldstein DS1, Jinsmaa Y2, Sullivan P2, et al. N-Acetylcysteine Prevents the Increase in Spontaneous Oxidation of Dopamine During Monoamine Oxidase Inhibition in PC12 Cells. Neurochem Res. 2017 Aug 24.

[2] Wright DJ1,2, Gray LJ3,2, Finkelstein DI1, et al. N-acetylcysteine modulates glutamatergic dysfunction and depressive behavior in Huntington's disease. Hum Mol Genet. 2016 Jul 15;25(14):2923-2933.

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Chemical Properties

Cas No. 616-91-1 SDF
别名 N-acetylcysteine; N-acetyl-L-cysteine; NAC; Acetadote
化学名 (2R)-2-acetamido-3-sulfanylpropanoic acid
Canonical SMILES CC(=O)NC(CS)C(=O)O
分子式 C5H9NO3S 分子量 163.19
溶解度 ≥8.2mg/mL in DMSO 储存条件 Store at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

产品描述


Acetylcysteine is a mucolytic agent which reduces the thickness of the mucus. Acetylcysteine is a ROS inhibitor[1].
Acetylcysteine prevents apoptotic DNA fragmentation and maintains long-term survival in the absence of other trophic support in serum-deprived PC12 cells. Acetylcysteine also prevents death of PC12 cells and sympathetic neurons[2]. Acetylcysteine causes dose-dependent reductions in viability in rat and human aortic smooth muscle cells[3]. Acetylcysteine activates the Ras-extracellular signal-regulated kinase (ERK) pathway in PC12 cells. Acetylcysteine protects neuronal cells from death evoked by withdrawal of trophic support. Acetylcysteine increases nitric oxide (NO) release from protein-bound stores in vascular tissue. Acetylcysteine pretreatment of PC12 cells interferes with NGF-dependent signaling and neurite outgrowth, and it is suggested that Acetylcysteine interferes with redox-sensitive steps in the NGF mechanism[4].
Acetylcysteine (150, 300 mg/kg) treatment significantly reduces liver transaminases in all groups of treatment, mostly in group Acetylcysteine 300. Lung glutathione peroxidase is significantly increases in group Acetylcysteine 300 (P=0.04), while the other oxidation biomarkers show no significant differences[6]. Acetylcysteine improves cognition of 12-month-old SAMP8 mice in both the T-maze footshock avoidance paradigm and the lever press appetitive task without inducing non-specific effects on motor activity, motivation to avoid shock, or body weight[5].
Reference:
[1]. Halasi M, et al. ROS inhibitor N-acetyl-L-cysteine antagonizes the activity of proteasome inhibitors. Biochem J. 2013 Sep 1;454(2):201-8.
[2]. Ferrari G, et al. N-acetylcysteine (D- and L-stereoisomers) prevents apoptotic death of neuronal cells. J Neurosci. 1995 Apr;15(4):2857-66.
[3]. Tsai JC, et al. Induction of apoptosis by pyrrolidinedithiocarbamate and N-acetylcysteine in vascular smooth muscle cells. J Biol Chem. 1996 Feb 16;271(7):3667-70.
[4]. Yan CY, et al. Prevention of PC12 cell death by N-acetylcysteine requires activation of the Ras pathway. J Neurosci. 1998 Jun 1;18(11):4042-9.
[5]. Farr SA, et al. The antioxidants alpha-lipoic acid and N-acetylcysteine reverse memory impairment and brain oxidative stress in aged SAMP8 mice. J Neurochem. 2003 Mar;84(5):1173-83.
[6]. Kalimeris K, et al. N-acetylcysteine ameliorates liver injury in a rat model of intestinal ischemia reperfusion. J Surg Res. 2016 Dec;206(2):263-272
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