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8-iso Prostaglandin F2α-d4

(Synonyms: iPF2αIIId4, 8Isoprostaned4, 15F2tIsoprostaned4, 8epi PGF2αd4) 目录号 : GC46747

An internal standard for the quantification of 8-iso prostaglandin F

8-iso Prostaglandin F2α-d4 Chemical Structure

Cas No.:211105-40-7

规格 价格 库存 购买数量
25 μg
¥2,296.00
现货
50 μg
¥4,369.00
现货
100 μg
¥8,258.00
现货
500 μg
¥36,729.00
现货

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Sample solution is provided at 25 µL, 10mM.

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产品描述

8-iso PGF-d4 contains four deuterium atoms at the 3, 3', 4, and 4' positions. It is intended for use as an internal standard for the quantification of 8-iso PGF by GC- or LC-mass spectrometry. 8-iso Prostaglandin F (8-iso PGF) is an isoprostane produced by the non-enzymatic peroxidation of arachidonic acid in membrane phospholipids.1,2,3 It circulates in human plasma in two distinct forms - esterified in LDL phospholipids and as the free acid. The ratio of these two forms is approximately 2:1, with a total plasma 8-iso PGF level of about 150 pg/ml in normal volunteers. In normal human urine, 8-iso PGF levels are about 180-200 pg/mg of creatinine.1,2 8-iso PGF is a weak TP receptor agonist in vascular smooth muscle.4 Conversely, 8-iso PGF inhibits platelet aggregation induced by U-46619 (10-6 M) and I-BOP (3 x 10-7 M) with IC50 values of 1.6 x 10-6 M and 1.8 x 10-6 M, respectively.3

1.Morrow, J.D., Hill, K.E., Burk, R.F., et al.A series of prostaglandin F2-like compounds are produced in vivo in humans by a non-cyclooxygenase, free radical-catalyzed mechanismProc. Natl. Acad. Sci. U.S.A.87(23)9383-9387(1990) 2.Morrow, J.D., Harris, T.M., and Roberts, L.J., IINoncyclooxygenase oxidative formation of a series of novel prostaglandins: Analytical ramifications for measurement of eicosanoidsAnal. Biochem.184(1)1-10(1990) 3.Morrow, J.D., Minton, T.A., and Roberts, L.J., IIThe F2-isoprostane, 8-epi-prostaglandin F2α, a potent agonist of the vascular thromboxane/endoperoxide receptor, is a platelet thromboxane/endoperoxide receptor antagonistProstaglandins44(2)155-163(1992) 4.Kiriyama, M., Ushikubi, F., Kobayashi, T., et al.Ligand binding specificities of the eight types and subtypes of the mouse prostanoid receptors expressed in Chinese hamster ovary cellsBr. J. Pharmacol.122(2)217-224(1997)

Chemical Properties

Cas No. 211105-40-7 SDF
别名 iPF2αIIId4, 8Isoprostaned4, 15F2tIsoprostaned4, 8epi PGF2αd4
Canonical SMILES O[C@@H]1[C@@H](C/C=C\C([2H])([2H])C([2H])([2H])CC(O)=O)[C@@H](/C=C/[C@H](O)CCCCC)[C@H](O)C1
分子式 C20H30D4O5 分子量 358.5
溶解度 10 mM Na2CO3: >6.5 mg/ml (from PGF2a),DMF: >100 mg/ml (from PGF2a),DMSO: >100 mg/ml (from PGF2a),Ethanol: >100 mg/ml (from PGF2a),PBS pH 7.2: >10 mg/ml (from PGF2a) 储存条件 Store at -20°C
General tips 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

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1 mg 5 mg 10 mg
1 mM 2.7894 mL 13.947 mL 27.894 mL
5 mM 0.5579 mL 2.7894 mL 5.5788 mL
10 mM 0.2789 mL 1.3947 mL 2.7894 mL
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