8-Bromo-cAMP, sodium salt
(Synonyms: 8-溴腺苷-3',5'-环单磷酸钠,8-Br-Camp sodium salt) 目录号 : GC16929
A PKA activator
Cas No.:76939-46-3
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
| Cell experiment [1]: | |
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Cell lines |
Growth-arrested AML193 cells |
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Preparation method |
The solubility of this compound in sterile water is 100 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months. |
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Reacting condition |
0 ~ 15 μM |
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Applications |
In growth-arrested AML193 cells, 8-Bromo-cAMP significantly increased DNA synthesis, with the ED50 value of approximately 10 μM. At the doses over 2.5 μM, a toxic effect was observed. Moreover, the addition of 1 μM 8-Bromo-cAMP synergized the IL-3-induced mitogenic response. In addition, 8-Bromo-cAMP at the concentration of 1μM resulted in maximal Erk phosphorylation. But the effect of 8-Bromo-cAMP on Erk phosphorylation was transient, with the maximal stimulation shown between 1 and 5 mins. |
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References: [1]. Rene e M. Y. Barge, J.H. Frederik Falkenburg, etal., 8-Bromo-cAMP induces a proliferative response in an IL-3 dependent leukemic cell line and activates Erk1,2 via a Shc-independent pathway. Biochimica et Biophysica Acta 1355 (1997) 141–146. |
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8-Bromo-cAMP is a cAMP-analogues. [1]
cAMP can have an effect on the proliferative response of a cell. Either the effect is a positive or a negative regulator depending on the cell types. There is also variable effect of cAMP in hematopoietic cell lines. CAMP-analogues can significantly inhibit the erythropoietin-stimulated proliferation.[1]
8-Bromo-cAMP increased the cellular content of mRNA encoding the hCG a- and β-subunits and prevented the increase in fibronectin mRNA. This is determined by blot hybridization analysis using specific cDNA probes. 8-Bromo-cAMP also induced phosphorylation of Erk1,2 in AML193 cells. 8-Bromo-cAMP is an agent in AML193 cells and activates Erk1,2 , this condition happens does not accompanied by the involvement of Shc phosphorylation.[1,2]
8-bromo-cAMP increases catecholamine biosynthesis and produces an increase both in the activity and phosphorylation of tyrosine hydroxylase. 8-bromo-cAMP also promotes alterations in the synthesis and secretion of specific proteins, including fibronectin and the subunits of hCG by regulating mRNA expression.[2,3]
References:
[1]Ulloa-Aguirre A,?August AM,?etal. , 8-Bromo-adenosine?3',5'-monophosphate?regulates?expression?of?chorionic?gonadotropin?and fibronectin in human cytotrophoblasts. J Clin Endocrinol Metab.?1987 May;64(5):1002-9.
[2] Rene e M. Y. Barge, J.H. Frederik Falkenburg, etal., 8-Bromo-cAMP induces a proliferative response in an IL-3 dependent leukemic cell line and activates Erk1,2 via a Shc-independent pathway. Biochimica et Biophysica Acta 1355 (1997) 141–146.
[3] Haycock JW, Bennett WF, etal. , Multiple site phosphorylation of tyrosine hydroxylase. Differential regulation in situ by a 8-bromo-cAMP and acetylcholine. J Biol Chem. 1982 Nov 25;257(22):13699-703.
| Cas No. | 76939-46-3 | SDF | |
| 别名 | 8-溴腺苷-3',5'-环单磷酸钠,8-Br-Camp sodium salt | ||
| 化学名 | sodium (4aR,6R,7R,7aS)-6-(6-amino-8-bromo-9H-purin-9-yl)-7-hydroxytetrahydro-4H-furo[3,2-d][1,3,2]dioxaphosphinin-2-olate 2-oxide | ||
| Canonical SMILES | NC1=C(N=C(Br)N2[C@@H]3O[C@H](COP(O4)([O-])=O)[C@@H]4[C@H]3O)C2=NC=N1.[Na+] | ||
| 分子式 | C10H10BrN5NaO6P | 分子量 | 430.09 |
| 溶解度 | ≥ 43mg/mL in Water;DMSO : 250 mg/mL (581.29 mM; Need ultrasonic) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.3251 mL | 11.6255 mL | 23.2509 mL |
| 5 mM | 0.465 mL | 2.3251 mL | 4.6502 mL |
| 10 mM | 0.2325 mL | 1.1625 mL | 2.3251 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。