| 6-diazo-5-oxo-L-nor-Leucine 目录号 GC41224 |
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
6-diazo-5-oxo-L-nor-Leucine Dilution Calculator
6-diazo-5-oxo-L-nor-Leucine Molarity Calculator
| Cas No. | 157-03-9 | SDF | Download SDF |
| 别名 | DON, NSC 7365O | ||
| 化学名 | 6-diazo-5-oxo-L-norleucine | ||
| Canonical SMILES | OC([C@@H](N)CCC(C=[N+]=[N-])=O)=O | ||
| 分子式 | C6H9N3O3 | 分子量 | 171.2 |
| 溶解度 | 1mg/mL in DMSO | 储存条件 | Store at -20°C |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
| Shipping Condition | Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request |
||
6-Diazo-5-oxo-L-nor-Leucine (DON) is a glutamine analog that inhibits glutaminases (Ki = 6 µM) and other glutamine-utilizing enzymes, including cytidine triphosphate synthase (CTPS), which uses glutamine in the synthesis of CTP.[1],[2],[3] DON is commonly used in cells or tissues but its use in whole animals is limited by its teratogenicity.[3],[4],[5]
Reference:
[1]. Willis, R.C., and Seegmiller, J.E. The inhibition by 6-diazo-5-oxo-l-norleucine of glutamine catabolism of the cultured human lymphoblast. J.Cell Physiol. 93, 375-382 (1977).
[2]. Thangavelu, K., Chong, Q.Y., Low, B.C., et al. Structural basis for the active site inhibition mechanism of human kidney-type glutaminase (KGA). Sci.Rep. 4, 1-7 (2014).
[3]. Thomas, A.G., Rojas, C., Tanega, C., et al. Kinetic characterization of ebselen, chelerythrine and apomorphine as glutaminase inhibitors. Biochemical and Biophysical Research Communications 438(2), 243-248 (2013).
[4]. Botman, D., Tigchelaar, W., and Van Noorden, C.J.F. Determination of phosphate-activated glutaminase activity and its kinetics in mouse tissues using metabolic mapping (quantitative enzyme histochemistry). Journal of Histochemistry and Cytochemistry 62(11), 813-826 (2014).
[5]. Greene, R.M., and Kochhar, D.M. Limb development in mouse embryos: Protection against teratogenic effects of 6-diazo-5-ox-L-norleucine (DON) in vivo and in vitro. J.Embryol.Exp.Morphol. 33(2), 355-370 (1975).