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5-bromo-5-nitro-1,3-Dioxane Sale

(Synonyms: 5-溴-5-硝基-1,3-二噁烷) 目录号 : GC42492

An antimicrobial agent

5-bromo-5-nitro-1,3-Dioxane Chemical Structure

Cas No.:30007-47-7

规格 价格 库存 购买数量
1g
¥686.00
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5g
¥1,199.00
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10g
¥2,055.00
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25g
¥3,426.00
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Sample solution is provided at 25 µL, 10mM.

产品文档

Quality Control & SDS

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产品描述

5-bromo-5-nitro-1,3-Dioxane is an antimicrobial compound that is effective against Gram-positive and Gram-negative bacteria and fungi, including yeast. Its mode of action occurs via the oxidation of essential protein thiols causing inhibition of enzyme activity and subsequent inhibition of microbial growth. This compound has been used as a preservative for biological molecules and solutions such as antibodies and antisera.

Chemical Properties

Cas No. 30007-47-7 SDF
别名 5-溴-5-硝基-1,3-二噁烷
Canonical SMILES BrC1([N+]([O-])=O)COCOC1
分子式 C4H6BrNO4 分子量 212
溶解度 DMF: 30 mg/ml,DMSO: 30 mg/ml,DMSO:PBS(pH 7.2) (1:4): 0.2 mg/ml,Ethanol: 25 mg/ml 储存条件 Store at -20°C
General tips 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

溶解性数据

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1 mg 5 mg 10 mg
1 mM 4.717 mL 23.5849 mL 47.1698 mL
5 mM 0.9434 mL 4.717 mL 9.434 mL
10 mM 0.4717 mL 2.3585 mL 4.717 mL
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Research Update

Mode of action of the antimicrobial compound 5-bromo-5-nitro-1,3-Dioxane (bronidox)

Folia Microbiol (Praha) 1986;31(1):19-31.PMID:3082729DOI:10.1007/BF02928676.

The mode of action of the antimicrobial agent, 5-bromo-5-nitro-1,3-Dioxane (bronidox), was studied in detail for gram-positive and gram-negative bacteria, yeast and fungi. The studies included MIC testing, thiol inhibition of activity, intracellular leakage, oxygen consumption, incorporation of 3H-uridine, scanning electron microscopy, inhibition of enzyme activity (papain) and in vitro oxidation of thiols to disulfides. It appears that the primary mode of action of bronidox is the same as, or similar to, that of bronopol, i.e. the oxidation of essential protein thiol causing inhibition of enzyme activity and subsequent inhibition of microbial growth.

Development of a solid-phase microextraction gas chromatography with microelectron-capture detection method for the determination of 5-bromo-5-nitro-1,3-Dioxane in rinse-off cosmetics

J Chromatogr A 2010 Oct 22;1217(43):6634-9.PMID:20444463DOI:10.1016/j.chroma.2010.04.027.

5-bromo-5-nitro-1,3-Dioxane (bronidox) is a bromine-containing preservative often used in rinse-off cosmetics but also subjected to several restrictions according to the European Cosmetic Products Regulation. Thus, as a part of a quality control procedure, analytical methods for the determination of this compound in different types of cosmetics are required. In the present work, a solvent-free and simple methodology based on solid-phase microextraction (SPME) followed by gas chromatography with microelectron capture detection (GC-μECD) has been developed and validated for the determination of bronidox in cosmetic samples such as shampoos, body cleansers or facial exfoliants. As far as we know, this is the first application of SPME to this preservative. Negative matrix effects due to the complexity of the studied samples were reduced by dilution with ultrapure water. The influence of several factors on the SPME procedure such as fiber coating, extraction temperature, salt addition (NaCl) and sampling mode has been assessed by performing a 2(4)-factorial design. After optimization, the recommended procedure was established as follows: direct solid-phase microextraction (DSPME), using a PDMS/DVB coating, of 10 mL of diluted cosmetic with 20% NaCl, at room temperature, under stirring for 30 min. Using these suggested extraction conditions, linear calibration could be achieved, with limits of detection (LOD) and quantification (LOQ) well below the maximum authorized concentration (0.1%) established by the European legislation. Relative standard deviations (RSD) lower than 10% were obtained for both within a day and among days precision. The method was applied to diverse types of formulations spiked with bronidox at different concentration levels (0.008-0.10%); these samples were quantified by external calibration and satisfactory recoveries (≥ 70%) were obtained in all cases. Finally, the SPME-GC-μECD methodology was applied to the analysis of several cosmetics labeled or not as containing bronidox. The presence of this preservative in some of these samples was confirmed by GC-MS.