5,15-Diacetyl-3-benzoyllathyrol
(Synonyms: 二乙酰基苯甲酰基香豌豆醇,Euphorbia factor L3) 目录号 : GN10153
5,15-Diacetyl-3-benzoyllathyrol 是一种具有抗癌活性的二萜类化合物。
Cas No.:218916-52-0
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cell experiment: | Cells are harvested during logarithmic growth phase and seeded in 96-well plates at a density of 1.5×104 cells/mL of final volume 190 μL/well. After incubation of 24 h, 10 μL EFL3 solution of full range concentrations is added to 96-well plates. After 68 h treatment, 10 μL MTT of 10 mM is added to each well for 4 h of maintaining at 37°C. Then, the supernatant is removed and the crystals are dissolved with 100 μL anhydrous DMSO each well. Subsequently, cell viability is measured by Model 550 Microplate reader at 540 nm and 655 nm as reference filter. Experiments are carried out at least thrice. The 50% inhibitory concentration (IC50) is defined as the anticancer agent concentration causing 50% reduction in cell viability and calculated from the cytotoxicity curves. Cell survival is calculated with the following formula: survival (%)=(mean experimental absorbance/mean control absorbance) × 100%. |
References: [1]. Zhang JY, et al. Structure identification of Euphorbia factor L3 and its induction of apoptosis through the mitochondrial pathway. Molecules. 2011 Apr 15;16(4):3222-31. | |
5,15-Diacetyl-3-benzoyllathyrol is one of the lathyrane diterpenoids, that has anti-cancer activity.
5,15-Diacetyl-3-benzoyllathyrol shows anticancer activity in vitro against lung cancer A549 cells and the IC50 values are 34.04±3.99 μM. Furthermore, 5,15-Diacetyl-3-benzoyllathyrol can induce apoptosis in A549 cells via the mitochondrial pathway including loss of mitochondrial potential and release of cytochrome c[1].
References:
[1]. Zhang JY, et al. Structure identification of Euphorbia factor L3 and its induction of apoptosis through the mitochondrial pathway. Molecules. 2011 Apr 15;16(4):3222-31.
| Cas No. | 218916-52-0 | SDF | |
| 别名 | 二乙酰基苯甲酰基香豌豆醇,Euphorbia factor L3 | ||
| Canonical SMILES | CC1CC2(C(C1OC(=O)C3=CC=CC=C3)C(C(=C)CCC4C(C4(C)C)C=C(C2=O)C)OC(=O)C)OC(=O)C | ||
| 分子式 | C31H38O7 | 分子量 | 522.63 |
| 溶解度 | ≥ 52.3mg/mL in DMSO | 储存条件 | 4°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.9134 mL | 9.567 mL | 19.134 mL |
| 5 mM | 0.3827 mL | 1.9134 mL | 3.8268 mL |
| 10 mM | 0.1913 mL | 0.9567 mL | 1.9134 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。