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4-oxo-2-Nonenal Alkyne Sale

(Synonyms: 4-ONE Alkyne, Alkynyl 4-oxo-2-Nonenal, Click Tag 4-oxo-2-Nonenal Alkyne) 目录号 : GC40498

An alkyne-tagged lipid peroxidation product

4-oxo-2-Nonenal Alkyne Chemical Structure

Cas No.:1680193-58-1

规格 价格 库存
100μg
¥839.00
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500μg
¥3,786.00
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1mg
¥6,716.00
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5mg
¥25,610.00
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Sample solution is provided at 25 µL, 10mM.

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产品描述

4-oxo-2-Nonenal is a product of lipid peroxidation that actively modifies histidine and lysine residues on proteins and causes protein cross-linking. It also modifies 2'-deoxyguanosine, further implicating lipid peroxidation in mutagenesis and carcinogenesis. 4-ONE alkyne is an alkyne-tagged electrophile that can be used as a probe to isolate and identify the reaction products of lipid peroxidation using click chemistry.

Chemical Properties

Cas No. 1680193-58-1 SDF
别名 4-ONE Alkyne, Alkynyl 4-oxo-2-Nonenal, Click Tag 4-oxo-2-Nonenal Alkyne
Canonical SMILES [H]C(/C=C/C(CCCC#C)=O)=O
分子式 C9H10O2 分子量 150.2
溶解度 DMF: 1 mg/ml,DMSO: 1 mg/ml,Ethanol: 10 mg/ml,PBS (pH 7.2): 5 mg/ml 储存条件 Store at -20°C
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储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

溶解性数据

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1 mg 5 mg 10 mg
1 mM 6.6578 mL 33.2889 mL 66.5779 mL
5 mM 1.3316 mL 6.6578 mL 13.3156 mL
10 mM 0.6658 mL 3.3289 mL 6.6578 mL
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Research Update

An ultrasensitive electrochemical aptasensor for the determination of tumor exosomes based on click chemistry

Biosens Bioelectron 2019 Oct 1;142:111503.PMID:31376716DOI:10.1016/j.bios.2019.111503.

Exosomes, lipid bilayer membrane vesicles, can guide various pathological and physiological processes. However, reliable, convenient and sensitive methods for exosome determination for early cancer diagnosis are still technically challenging. Herein, an electrochemical aptasensor based on click chemistry and the DNA hybridization chain reaction (HCR) for signal amplification has been developed for the ultrasensitive detection of tumor exosomes. CD63 aptamer was first immobilized on a glassy carbon electrode for capturing exosomes, and 4-oxo-2-Nonenal Alkyne (alkynyl-4-ONE) molecules, functionalized lipid electrophiles, were conjugated to the exosomes via the reaction of amino and aldehyde groups. Azide-labeled DNA probe as an anchor was then connected to the exosomes by copper (I)-catalyzed click chemistry. Signal amplification was achieved by HCR, and the numerous linked horseradish peroxidase (HRP) molecules could catalyze the reaction of o-phenylenediamine (OPD) and H2O2. The concentration of exosomes could be quantified by monitoring the electrochemical reduction current of 2,3-diaminophenazine (DAP). Under the optimal conditions, this method allowed the sensitive detection of exosomes in the range of 1.12 × 102 to 1.12 × 108 particles/μL with a limit of detection (LOD) of 96 particles/μL. Furthermore, the present assay enabled sensitive and accurate quantification of exosomes in human serum, and it has high potential for exosome analysis in clinical samples.