2-hydroxy Myristic Acid
(Synonyms: 2-羟基十四烷酸) 目录号 : GC42166A hydroxy fatty acid
Cas No.:2507-55-3
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
2-hydroxy Myristic acid is a hydroxy fatty acid that has been found in bovine, human, and horse milk, cow and buffalo cheeses, sea bass filet, seal oil, human vernix caseosa, and wool wax. It inhibits cleavage between the enterovirus capsid proteins VP4 and VP2, a process required for enterovirus infectivity, as well as Junin and Tacaribe viral replication (IC50s = 20.1 and 14.2 μM, respectively). 2-hydroxy Myristic acid was previously characterized as a weak inhibitor of peptide myristoylation (Ki = 200 μM) but has been shown to be inactive in ARL1 cells when used at 100 μM.
| Cas No. | 2507-55-3 | SDF | |
| 别名 | 2-羟基十四烷酸 | ||
| Canonical SMILES | CCCCCCCCCCCCC(O)C(=O)O | ||
| 分子式 | C14H28O3 | 分子量 | 244.4 |
| 溶解度 | DMF: 30 mg/ml,DMSO: 27 mg/ml,Ethanol: 37 mg/ml,Ethanol:PBS (1:2): 0.35 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.0917 mL | 20.4583 mL | 40.9165 mL |
| 5 mM | 0.8183 mL | 4.0917 mL | 8.1833 mL |
| 10 mM | 0.4092 mL | 2.0458 mL | 4.0917 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Isolation of a novel sphingoglycolipid containing glucuronic acid and 2-hydroxy fatty acid from Flavobacterium devorans ATCC 10829
J Biochem 1978 Apr;83(4):1213-6.PMID:659393DOI:10.1093/oxfordjournals.jbchem.a132015.
A new acidic sphingoglycolipid has been isolated from a Gram-negative, glucose-non-fermentative (obligatory aerobic) bacterium, Flavobacterium devorans ATCC 10829, by thin-layer chromatography on silica gel after mild alkaline hydrolysis of the cellular lipids. Chemical degradation studies, thin-layer chromatographic behavior, IR and mass-spectrometric analysis of the original and reduced glycolipid with LiA1H4 revealed that the lipid contained glucuronic acid, long-chain bases, and fatty acids in a molar ratio of approximately 1:1:1. The major long-chain bases were identified by gas chromatography-mass spectrometry as dihydrosphingosine (d-18 :0) and longer homologues, while the N-acyl group was exclusively 2-hydroxy Myristic Acid. The most probable structure of this glycolipid appeared to be a ceramide glucuronic acid (N-acyl dihydrosphingosine 1-glucuronic acid).
Isolation of an unusual 'lipid A' type glycolipid from Pseudomonas paucimobilis
Biochim Biophys Acta 1982 Sep 14;712(3):571-5.PMID:7126625DOI:10.1016/0005-2760(82)90285-5.
A new glycolipid was isolated from defatted cells of Pseudomonas paucimobilis IAM 12576, and called 'bound lipid'. The 'bound lipid' could not be extracted by hot phenol extraction, but could be extracted with hot chloroform/methanol after hydrolysis with 5% trichloroacetic acid. The 'bound lipid' was purified by thin-layer chromatography on silica gel plates using the solvent mixture CHCl3/CH3OH/CH3COOOH/ H2O (25:15:4:2, v/v). It consisted of glucosamine, 2-hydroxy Myristic Acid, galactose, mannose and uronic acid with ratios of 1.0:0.75:0.77:0.44:1.5, respectively, and other fatty acids besides 2-hydroxy Myristic Acid were not detected in the 'bound lipid'. 2-hydroxy Myristic Acid was probably bound to glucosamine residues by amide linkage, because mild alkali treatment did not liberate the fatty acid. From these results, we discussed the possibility that the 'bound lipid' was some kind of lipid A of this bacteria.
Stimulation of phagocytosis and phagosome-lysosome fusion by glycosphingolipids from Sphingomonas paucimobilis
J Biochem 1995 Aug;118(2):271-7.PMID:8543558DOI:10.1093/oxfordjournals.jbchem.a124902.
Sphingomonas paucimobilis, a Gram-negative opportunistic pathogen, is actively phagocytosed by human peripheral polymorphonuclear leukocytes (PMN) in vitro. However, when live or killed cells were delipidated, the phagocytic rate was clearly decreased. Therefore, we have investigated the physiological role of membrane lipids in phagocytic processes. S. paucimobilis type strain 2395 produces four classes of acidic glycosphingolipids (GL-1, GL-2, GL-3, and GL-4) with the common components of glucuronic acid, 2-hydroxy Myristic Acid and d18:0 or d21:1 long-chain base. The effect of acidic glycosphingolipids on phagocytosis by PMN using killed Staphylococcus aureus cells coated with glucuronosyl ceramide (GL-1) or ceramide tetrahexoside (GL-4) was also examined. The rate of phagosome-lysosome fusion by PMN was determined by counting acridine orange-stained bacteria under a fluorescence microscope. Both phagocytosis and phagosome-lysosome fusion by PMN of glycosphingolipid-coated bacteria were stimulated markedly in a dose-dependent manner. It was noted that GL-1 or GL-4 stimulated phagosome-lysosome fusion dramatically, but synthetic lipid A did not. Superoxide anion release from PMN was enhanced significantly by the coating with synthetic lipid A at higher concentration, but only slightly with GL-1 or GL-4. Glucuronic acid was an inhibitor of phagocytosis of GL-1-coated S. aureus by PMN. The effect of acidic glycosphingolipids obtained from mammalian tissue on phagocytosis was also compared with that of bacterial glycosphingolipids. Ganglioside GM3 and sulfatide showed a marked stimulative activity for phagocytosis by PMN, while the neutral glycosphingolipids did not. Thus, bacterial acidic glycosphingolipid and mammalian acidic glycosphingolipid promote phagocytosis and phagosome-lysosome fusion by PMN.